Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   MKD39_RS12365 Genome accession   NZ_CP092478
Coordinates   2525005..2525517 (-) Length   170 a.a.
NCBI ID   WP_011276866.1    Uniprot ID   A0A5B2YXI2
Organism   Staphylococcus haemolyticus strain CGMH-SH51     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 2499343..2524609 2525005..2525517 flank 396


Gene organization within MGE regions


Location: 2499343..2525517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKD39_RS12230 (MKD39_12210) - 2499354..2501022 (-) 1669 Protein_2377 IS1182 family transposase -
  MKD39_RS12235 (MKD39_12215) fabI 2501308..2502078 (-) 771 WP_000609031.1 enoyl-ACP reductase FabI -
  MKD39_RS12240 (MKD39_12220) - 2502592..2503266 (+) 675 WP_001105986.1 IS6-like element IS257 family transposase -
  MKD39_RS12245 (MKD39_12225) qac 2503371..2503694 (+) 324 WP_033080077.1 QacCGHJ group quaternary ammonium compound efflux SMR transporter -
  MKD39_RS12250 (MKD39_12230) - 2504378..2505034 (-) 657 WP_033080078.1 replication protein -
  MKD39_RS12255 (MKD39_12235) - 2505144..2505818 (-) 675 WP_010775116.1 IS6-like element IS257 family transposase -
  MKD39_RS12260 (MKD39_12240) - 2505862..2506281 (+) 420 WP_051607825.1 ParA family protein -
  MKD39_RS12265 (MKD39_12245) - 2506284..2506562 (+) 279 WP_000774717.1 hypothetical protein -
  MKD39_RS12270 (MKD39_12250) - 2506690..2507325 (+) 636 Protein_2385 aminoglycoside 6-adenylyltransferase -
  MKD39_RS12275 (MKD39_12255) sat4 2507322..2507864 (+) 543 WP_037570490.1 streptothricin N-acetyltransferase Sat4 -
  MKD39_RS12280 (MKD39_12260) aph(3')-IIIa 2507957..2508751 (+) 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
  MKD39_RS12755 - 2509027..2509158 (+) 132 Protein_2388 HTH domain-containing protein -
  MKD39_RS12760 - 2509234..2509793 (+) 560 Protein_2389 helix-turn-helix transcriptional regulator -
  MKD39_RS12290 (MKD39_12270) - 2509852..2510526 (-) 675 WP_010775116.1 IS6-like element IS257 family transposase -
  MKD39_RS12295 (MKD39_12275) - 2511378..2512112 (+) 735 WP_051607842.1 replication initiation protein -
  MKD39_RS12300 (MKD39_12280) - 2512133..2512807 (-) 675 WP_010775116.1 IS6-like element IS257 family transposase -
  MKD39_RS12305 (MKD39_12285) - 2512875..2513603 (+) 729 WP_011276853.1 ABC transporter ATP-binding protein -
  MKD39_RS12310 (MKD39_12290) - 2513600..2514685 (+) 1086 WP_011276854.1 ABC transporter permease -
  MKD39_RS12315 (MKD39_12295) - 2515053..2515154 (-) 102 WP_011276856.1 type I toxin-antitoxin system Fst family toxin -
  MKD39_RS12320 (MKD39_12300) isaB 2515516..2516001 (-) 486 WP_011276857.1 immunodominant staphylococcal antigen IsaB family protein -
  MKD39_RS12325 (MKD39_12305) - 2516204..2517902 (-) 1699 Protein_2397 IS1182 family transposase -
  MKD39_RS12330 (MKD39_12310) - 2518236..2519550 (+) 1315 Protein_2398 ISL3 family transposase -
  MKD39_RS12335 (MKD39_12315) - 2519679..2519927 (-) 249 WP_011276862.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  MKD39_RS12340 (MKD39_12320) - 2520105..2520854 (-) 750 Protein_2400 amidohydrolase family protein -
  MKD39_RS12345 (MKD39_12325) - 2521070..2521327 (+) 258 WP_011276864.1 hypothetical protein -
  MKD39_RS12350 (MKD39_12330) - 2521530..2522600 (+) 1071 WP_011276865.1 Ltp family lipoprotein -
  MKD39_RS12355 (MKD39_12335) - 2522912..2524592 (+) 1681 Protein_2403 IS1182 family transposase -
  MKD39_RS12360 (MKD39_12340) rpsR 2524715..2524957 (-) 243 WP_002447994.1 30S ribosomal protein S18 -
  MKD39_RS12365 (MKD39_12345) ssbA 2525005..2525517 (-) 513 WP_011276866.1 single-stranded DNA-binding protein Machinery gene

Sequence


Protein


Download         Length: 170 a.a.        Molecular weight: 18837.38 Da        Isoelectric Point: 4.7306

>NTDB_id=657954 MKD39_RS12365 WP_011276866.1 2525005..2525517(-) (ssbA) [Staphylococcus haemolyticus strain CGMH-SH51]
MINRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGEREADFINVVVFRRQAENVNNYLFKGSLAGVDGRIQSR
SYENQEGRRIFVTEVVADSVQFLEPKNAQGGQRNQSNNNDFQDYGQGFGGQQSGQNTSYNNNNSSNNKQSDNPFANANGP
IDISDDDLPF

Nucleotide


Download         Length: 513 bp        

>NTDB_id=657954 MKD39_RS12365 WP_011276866.1 2525005..2525517(-) (ssbA) [Staphylococcus haemolyticus strain CGMH-SH51]
ATGATAAACAGAGTTGTATTAGTAGGTCGTTTAACTAAAGATCCAGAATACAGAACCACTCCCTCAGGCGTAAGTGTAGC
GACATTTACTCTAGCAGTTAATCGTACTTTCACGAATGCTCAAGGGGAACGCGAAGCTGACTTCATCAATGTTGTTGTAT
TTAGAAGACAAGCAGAGAATGTGAATAATTATTTATTCAAAGGTAGTCTAGCTGGCGTTGATGGTCGCATACAATCACGC
AGTTATGAAAATCAAGAAGGTCGTCGTATCTTTGTTACTGAAGTTGTAGCAGATAGTGTTCAATTCCTTGAACCTAAAAA
TGCTCAAGGTGGCCAACGTAATCAAAGTAATAACAATGATTTCCAAGACTACGGTCAAGGATTTGGTGGTCAACAATCAG
GACAAAATACATCTTACAATAACAATAATTCATCAAATAATAAACAATCTGATAATCCATTTGCAAACGCAAACGGACCG
ATTGATATTAGTGATGATGACTTACCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A5B2YXI2

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

62.921

100

0.659

  ssb Latilactobacillus sakei subsp. sakei 23K

54.07

100

0.547

  ssbB Bacillus subtilis subsp. subtilis str. 168

60.377

62.353

0.376