NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	MJE83_RS14030	Genome accession	NZ_CP092446
Coordinates	2791318..2791932 (-)	Length	204 a.a.
NCBI ID	WP_015240245.1	Uniprot ID	-
Organism	Bacillus velezensis strain R-71003
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2786318..2796932

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MJE83_RS14000 (MJE83_14000)	rpsT	2786621..2786887 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
MJE83_RS14005 (MJE83_14005)	holA	2786904..2787945 (-)	1042	Protein_2729	DNA polymerase III subunit delta	-
MJE83_RS14010 (MJE83_14010)	-	2787985..2788136 (-)	152	Protein_2730	hypothetical protein	-
MJE83_RS14015 (MJE83_14015)	-	2788177..2788311 (+)	135	WP_003152870.1	YqzM family protein	-
MJE83_RS14020 (MJE83_14020)	comEC	2788330..2790681 (-)	2352	WP_015240244.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MJE83_RS14025 (MJE83_14025)	-	2790682..2791251 (-)	570	WP_003152868.1	ComE operon protein 2	-
MJE83_RS14030 (MJE83_14030)	comEA	2791318..2791932 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
MJE83_RS14035 (MJE83_14035)	comER	2791991..2792812 (+)	822	WP_015240246.1	late competence protein ComER	-
MJE83_RS14040 (MJE83_14040)	-	2792881..2793618 (-)	738	WP_015240247.1	class I SAM-dependent methyltransferase	-
MJE83_RS14045 (MJE83_14045)	rsfS	2793615..2793971 (-)	357	WP_007408260.1	ribosome silencing factor	-
MJE83_RS14050 (MJE83_14050)	yqeK	2793989..2794549 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
MJE83_RS14055 (MJE83_14055)	-	2794539..2795108 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
MJE83_RS14060 (MJE83_14060)	yhbY	2795119..2795409 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
MJE83_RS14065 (MJE83_14065)	aroE	2795403..2796245 (-)	843	WP_015240248.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22209.32 Da Isoelectric Point: 5.5211

                                >NTDB_id=657589 MJE83_RS14030 WP_015240245.1 2791318..2791932(-) (comEA) [Bacillus velezensis strain R-71003]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=657589 MJE83_RS14030 WP_015240245.1 2791318..2791932(-) (comEA) [Bacillus velezensis strain R-71003]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT

CCGTCGCGGACAAAAAAGACACAGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGGAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCAATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0657589