Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MJO57_RS00175 Genome accession   NZ_CP092417
Coordinates   46141..47253 (-) Length   370 a.a.
NCBI ID   WP_252021933.1    Uniprot ID   -
Organism   Endozoicomonas sp. SCSIO W0465     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 41141..52253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJO57_RS00170 (MJO57_00170) - 44092..46056 (+) 1965 WP_252021931.1 hypothetical protein -
  MJO57_RS00175 (MJO57_00175) pilU 46141..47253 (-) 1113 WP_252021933.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MJO57_RS00180 (MJO57_00180) - 47300..47785 (-) 486 WP_252021935.1 glutathione peroxidase -
  MJO57_RS00185 (MJO57_00185) - 48123..48290 (-) 168 WP_252021937.1 hypothetical protein -
  MJO57_RS00190 (MJO57_00190) - 48289..48714 (+) 426 WP_252021939.1 DUF6444 domain-containing protein -
  MJO57_RS00195 (MJO57_00195) - 48681..50339 (-) 1659 WP_252017470.1 transposase -
  MJO57_RS00200 (MJO57_00200) - 50372..51538 (+) 1167 WP_256493311.1 IS66 family transposase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41230.96 Da        Isoelectric Point: 5.8742

>NTDB_id=657282 MJO57_RS00175 WP_252021933.1 46141..47253(-) (pilU) [Endozoicomonas sp. SCSIO W0465]
MDINKALSLLADRNGSELFLTVGYPPCLKVNGQLVPIAKTALTSEQAHDIFEKMMGEERFKEFCETRESNYAMQNPDSGR
FRISAFFQRGEPGMVIRRIHHHIPSPQELGLPDIFCDLILQERGLILITGPAGAGKSTAMASLVNYRNHVGKGHIITIED
PVEFIHSHNQCIVTQREVGLDTQSYEEGLSNALRQAPDLVVIGEIRTPEVMRHTIEFVQTGHLCIATLHANTAYQALERI
IHFFPQEQHEEILLDLSLSLQGVMGQQLISSEDGTAAYPSHEILLNTPALADLIKKGKVDEIHDLMERSKDSGMQTIDQS
IFKLFKAGKITPQQAIQHADSSNNMRLLIKMHGHQVGGDDSFDHLTISTD

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=657282 MJO57_RS00175 WP_252021933.1 46141..47253(-) (pilU) [Endozoicomonas sp. SCSIO W0465]
GTGGATATCAACAAAGCGCTCTCCTTATTGGCTGATCGTAACGGTTCAGAACTGTTTTTAACGGTCGGCTATCCCCCCTG
TCTGAAAGTCAATGGCCAATTGGTGCCTATCGCCAAGACCGCTCTTACCAGCGAGCAGGCACATGATATTTTTGAAAAAA
TGATGGGTGAAGAGCGCTTTAAGGAATTCTGTGAGACTCGTGAGAGTAATTATGCTATGCAGAATCCGGACTCGGGTCGT
TTTCGTATCAGTGCATTCTTCCAAAGAGGGGAGCCCGGAATGGTGATCCGCCGCATTCACCATCATATTCCTTCCCCACA
GGAGCTCGGCCTACCCGATATTTTTTGTGACCTGATTCTTCAGGAAAGAGGTTTAATTCTGATTACCGGGCCAGCAGGAG
CGGGAAAATCAACCGCTATGGCATCACTGGTTAATTATCGAAACCATGTGGGTAAAGGTCATATCATCACCATTGAAGAC
CCTGTTGAATTTATCCACAGCCATAATCAGTGTATCGTCACCCAGCGAGAAGTTGGACTGGATACCCAATCTTATGAAGA
GGGTCTGTCAAACGCTCTACGTCAGGCACCGGACCTGGTGGTTATTGGCGAGATCCGTACCCCCGAGGTCATGAGGCATA
CCATTGAATTTGTTCAAACGGGTCACCTTTGTATCGCAACCCTTCATGCCAATACAGCCTATCAAGCGCTGGAACGGATC
ATTCACTTTTTTCCACAAGAACAACATGAAGAGATTCTTCTTGATTTGTCACTGAGCCTGCAGGGTGTTATGGGGCAGCA
GTTAATCAGCTCTGAAGATGGTACGGCCGCCTACCCATCCCATGAAATCCTGTTAAATACCCCCGCGCTGGCAGATCTGA
TCAAAAAGGGTAAAGTGGATGAAATTCATGACCTGATGGAGCGCTCTAAAGACTCGGGTATGCAAACCATTGATCAATCT
ATCTTCAAACTTTTCAAAGCTGGAAAAATTACCCCCCAGCAAGCTATACAGCATGCTGACTCCTCGAATAACATGCGCTT
GTTAATAAAGATGCATGGGCATCAAGTAGGAGGAGATGATTCTTTTGATCACTTAACCATCTCCACGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

49.727

98.919

0.492
  pilU Vibrio cholerae strain A1552

48.108

100

0.481
  pilU Acinetobacter baylyi ADP1

50.568

95.135

0.481