Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MID01_RS13455 Genome accession   NZ_CP092369
Coordinates   2591294..2591911 (-) Length   205 a.a.
NCBI ID   WP_041850029.1    Uniprot ID   -
Organism   Bacillus subtilis strain ZW     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2586294..2596911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MID01_RS13425 (MID01_13425) rpsT 2586596..2586862 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  MID01_RS13430 (MID01_13430) holA 2586877..2587920 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  MID01_RS13435 (MID01_13435) - 2587960..2588109 (-) 150 WP_120363468.1 hypothetical protein -
  MID01_RS13440 (MID01_13440) yqzM 2588150..2588284 (+) 135 WP_003229983.1 YqzM family protein -
  MID01_RS13445 (MID01_13445) comEC 2588324..2590654 (-) 2331 WP_041850028.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  MID01_RS13450 (MID01_13450) comEB 2590658..2591227 (-) 570 WP_003229978.1 ComE operon protein 2 -
  MID01_RS13455 (MID01_13455) comEA 2591294..2591911 (-) 618 WP_041850029.1 competence protein ComEA Machinery gene
  MID01_RS13460 (MID01_13460) comER 2591995..2592816 (+) 822 WP_041850030.1 late competence protein ComER -
  MID01_RS13465 (MID01_13465) yqeM 2592882..2593625 (-) 744 WP_032726197.1 class I SAM-dependent methyltransferase -
  MID01_RS13470 (MID01_13470) rsfS 2593622..2593978 (-) 357 WP_003237112.1 ribosome silencing factor -
  MID01_RS13475 (MID01_13475) yqeK 2593996..2594556 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MID01_RS13480 (MID01_13480) nadD 2594546..2595115 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  MID01_RS13485 (MID01_13485) yhbY 2595127..2595417 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  MID01_RS13490 (MID01_13490) aroE 2595411..2596253 (-) 843 WP_041850031.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21821.62 Da        Isoelectric Point: 4.7220

>NTDB_id=656864 MID01_RS13455 WP_041850029.1 2591294..2591911(-) (comEA) [Bacillus subtilis strain ZW]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEVQVNLAEILQDGIVVYIPKKGEETAVQQGGGVSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=656864 MID01_RS13455 WP_041850029.1 2591294..2591911(-) (comEA) [Bacillus subtilis strain ZW]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCAATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGTGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGATAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGTGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATATCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-117)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.049

100

0.98
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

35.455

100

0.38