Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MIS33_RS04595 Genome accession   NZ_CP092359
Coordinates   918015..920120 (+) Length   701 a.a.
NCBI ID   WP_249443787.1    Uniprot ID   -
Organism   Wielerella bovis strain 08LB461     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 913015..925120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS33_RS04575 (MIS33_04575) pilM 915052..916164 (+) 1113 WP_249443534.1 type IV pilus assembly protein PilM -
  MIS33_RS04580 (MIS33_04580) - 916166..916783 (+) 618 WP_249443535.1 PilN domain-containing protein -
  MIS33_RS04585 (MIS33_04585) pilO 916780..917424 (+) 645 WP_249443536.1 type 4a pilus biogenesis protein PilO -
  MIS33_RS04590 (MIS33_04590) - 917450..917977 (+) 528 WP_249443537.1 pilus assembly protein PilP -
  MIS33_RS04595 (MIS33_04595) pilQ 918015..920120 (+) 2106 WP_249443787.1 type IV pilus secretin PilQ Machinery gene
  MIS33_RS04600 (MIS33_04600) - 920173..920685 (+) 513 WP_249443538.1 shikimate kinase -
  MIS33_RS04605 (MIS33_04605) aroB 920695..921777 (+) 1083 WP_249446507.1 3-dehydroquinate synthase -
  MIS33_RS04610 (MIS33_04610) - 921807..922226 (+) 420 WP_249443540.1 ProQ/FINO family protein -
  MIS33_RS04615 (MIS33_04615) - 922344..922913 (+) 570 WP_249446506.1 peptidylprolyl isomerase -
  MIS33_RS04620 (MIS33_04620) - 923004..923513 (+) 510 WP_249443541.1 peptidylprolyl isomerase -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 76397.47 Da        Isoelectric Point: 9.4091

>NTDB_id=656718 MIS33_RS04595 WP_249443787.1 918015..920120(+) (pilQ) [Wielerella bovis strain 08LB461]
MKINSIKIMSAVALGLLFQAANAGSITDINVSVLPNKQRVIKFKFDSGVVEPSGFITSAPARIALDFPATDIKVSQSQLT
FNDQLLKQIIAAQGSEHARVLLGLGKEGQYSTEIKGNEVWVYLSEAESTNTAAVAAAQSVVSSAPKNSQSYQSSGITAPF
NIDFHKGANRSGVIEYSSNYSGQPQMRVQSDRLIILLKDYPLSVQDQRNLDVTDFSTPVRTINVRRIGNDAQIIIRSQGT
WEHKVSGRNGRQTIHIVPTKNISTIGLSRKNDQKFTGKNVSLDFQNVDVRTVLQILATESGMNIVASDNVTGKMTLSLKD
VPWDQALDLILDARNLDMRKVGNIINIAPRSDLLARDTEALEAQRKLDDLGPLISQSFQLKYKNVEEFRKILRISDSASV
SSDRNSILSSRGSALIDPATNTLIVTDVQSVITKFNKLIEELDVPSRQVMIEARIVEAADGFSRDLGVQFGYQRSGAHTS
VGSNWSNSINNANVGYGSATSSILNPNVALPSSASTSSIGIVRAVSSGALGLELSASESENRSKTISTPRVLTQDRQEAE
IRQGYQIPYTKREDGETSIEWKAAELVLKTTPRITPDNKVILDIEIKKDEPDGTYTAITGEAGLATRYVKTQAMIEDGGT
LVVGGIYQEATSNSIRKVPLLGDIPVVGNLFKARARKSSRNELLFFITPRIMGSETSVLRY

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=656718 MIS33_RS04595 WP_249443787.1 918015..920120(+) (pilQ) [Wielerella bovis strain 08LB461]
ATGAAAATAAATTCTATCAAAATAATGTCAGCAGTAGCATTAGGTTTATTATTTCAAGCTGCAAATGCGGGAAGTATTAC
TGATATTAATGTTTCGGTACTTCCAAATAAACAACGTGTAATTAAATTCAAGTTTGATAGTGGTGTGGTTGAACCTAGTG
GTTTTATTACATCAGCTCCTGCTCGTATTGCTTTGGATTTTCCTGCAACAGATATTAAAGTTTCACAATCTCAATTAACT
TTTAATGATCAACTGCTAAAACAGATTATTGCTGCACAAGGTAGTGAGCATGCTCGTGTACTGTTGGGGTTGGGTAAAGA
AGGACAATATAGTACTGAAATTAAAGGAAATGAGGTTTGGGTATATTTGAGTGAAGCTGAGTCAACTAATACAGCTGCAG
TTGCTGCTGCGCAATCTGTTGTAAGCTCGGCTCCTAAAAATTCACAATCTTACCAATCTTCTGGAATAACAGCGCCATTT
AATATTGATTTCCATAAAGGTGCAAATAGATCAGGTGTAATTGAGTATTCATCAAATTATTCAGGTCAGCCTCAAATGAG
AGTGCAAAGTGATCGATTGATTATCTTGTTAAAAGATTATCCATTGTCGGTTCAAGATCAACGTAATTTAGATGTAACCG
ATTTTTCTACTCCTGTGCGTACTATTAATGTTCGTCGTATTGGTAATGATGCGCAGATTATTATCAGAAGTCAGGGAACA
TGGGAGCATAAAGTTTCTGGTAGAAATGGACGCCAAACAATTCATATTGTTCCAACGAAAAATATTTCTACTATTGGGTT
AAGTCGGAAGAATGATCAGAAATTTACAGGAAAGAATGTTTCATTAGATTTCCAAAATGTTGATGTTCGTACTGTATTGC
AGATTCTAGCAACAGAGTCAGGAATGAATATTGTTGCGAGTGATAATGTAACAGGTAAAATGACATTGTCATTGAAAGAT
GTACCTTGGGATCAGGCTTTAGATTTGATTTTGGATGCACGCAATTTGGATATGCGTAAAGTTGGTAATATTATCAATAT
TGCACCGCGTTCAGATTTGTTGGCTCGTGATACAGAAGCACTTGAAGCTCAAAGAAAATTGGATGATTTAGGTCCTCTGA
TTTCTCAAAGTTTTCAATTGAAATATAAAAATGTTGAGGAATTTAGAAAAATTTTACGCATCAGTGATTCTGCATCAGTA
AGTTCTGATAGAAATTCAATCCTTAGTTCACGTGGTAGTGCGTTGATTGATCCTGCAACAAATACATTGATCGTTACCGA
TGTTCAATCTGTAATTACGAAATTTAATAAATTAATTGAAGAATTGGATGTTCCTTCTCGTCAAGTAATGATTGAAGCGC
GTATTGTTGAAGCTGCTGATGGATTTTCGCGTGATTTAGGTGTTCAATTTGGTTATCAGCGTTCAGGAGCACATACTTCT
GTAGGCTCAAATTGGTCTAATAGTATTAATAATGCGAATGTTGGTTATGGGTCGGCTACATCAAGTATTTTAAATCCAAA
TGTTGCATTACCTTCTTCTGCATCAACAAGTTCTATCGGTATTGTGCGAGCTGTGTCGTCAGGTGCATTGGGTTTGGAAT
TGTCGGCATCAGAGTCGGAAAATCGCAGTAAAACGATTTCTACGCCACGCGTATTAACTCAAGATCGTCAAGAAGCGGAA
ATTCGTCAAGGTTATCAGATTCCTTATACAAAAAGGGAAGATGGTGAAACTTCTATCGAATGGAAAGCTGCGGAATTGGT
CTTGAAAACAACACCTCGTATTACACCAGATAACAAAGTTATTTTGGATATTGAAATTAAAAAAGATGAACCTGATGGTA
CTTATACCGCAATCACAGGTGAAGCAGGTCTTGCAACACGTTATGTGAAAACACAAGCGATGATTGAAGATGGTGGTACA
TTGGTTGTGGGTGGTATTTATCAAGAAGCAACATCCAATTCAATTCGTAAAGTACCTTTACTGGGTGATATTCCAGTTGT
TGGTAATTTATTTAAAGCGCGAGCACGTAAAAGTTCACGCAATGAATTGCTGTTCTTCATTACACCGCGTATTATGGGTT
CTGAAACTAGCGTATTGCGTTACTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(376-448)

Bacterial type II and III secretion system protein (Secretin)

(536-692)

Secretin and TonB N terminus short domain (STN)

(303-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria gonorrhoeae strain FA1090

50.983

100

0.518
  pilQ Neisseria meningitidis M1080

48.133

100

0.515
  pilQ Neisseria meningitidis MC58

53.636

78.459

0.421