Detailed information NTG0656244

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MF598_RS07460
• Genome accession: NZ_CP092185
• Coordinates: 1438352..1438966 (+)
• Length: 204 a.a.
• NCBI ID: WP_045208286.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain K203
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1433352..1443966

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MF598_RS07425 (MF598_07425)	aroE	1434039..1434881 (+)	843	WP_032873946.1	shikimate dehydrogenase	-
MF598_RS07430 (MF598_07430)	yhbY	1434875..1435165 (+)	291	WP_045208288.1	ribosome assembly RNA-binding protein YhbY	-
MF598_RS07435 (MF598_07435)	-	1435176..1435745 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
MF598_RS07440 (MF598_07440)	yqeK	1435735..1436295 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
MF598_RS07445 (MF598_07445)	rsfS	1436313..1436669 (+)	357	WP_007408260.1	ribosome silencing factor	-
MF598_RS07450 (MF598_07450)	-	1436666..1437403 (+)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
MF598_RS07455 (MF598_07455)	comER	1437472..1438293 (-)	822	WP_012118027.1	late competence protein ComER	-
MF598_RS07460 (MF598_07460)	comEA	1438352..1438966 (+)	615	WP_045208286.1	helix-hairpin-helix domain-containing protein	Machinery gene
MF598_RS07465 (MF598_07465)	-	1439033..1439602 (+)	570	WP_003152868.1	ComE operon protein 2	-
MF598_RS07470 (MF598_07470)	comEC	1439603..1441954 (+)	2352	WP_045208284.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MF598_RS07475 (MF598_07475)	-	1441973..1442107 (-)	135	WP_003152870.1	YqzM family protein	-
MF598_RS07480 (MF598_07480)	-	1442148..1442299 (+)	152	Protein_1471	hypothetical protein	-
MF598_RS07485 (MF598_07485)	holA	1442339..1443380 (+)	1042	Protein_1472	DNA polymerase III subunit delta	-
MF598_RS07490 (MF598_07490)	rpsT	1443397..1443663 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22323.60 Da
• Isoelectric Point: 7.7754

>NTDB_id=656244 MF598_RS07460 WP_045208286.1 1438352..1438966(+) (comEA) [Bacillus velezensis strain K203]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTTDDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELKVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQVISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=656244 MF598_RS07460 WP_045208286.1 1438352..1438966(+) (comEA) [Bacillus velezensis strain K203]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCACAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGTCTGAAGAAAAAGGCGGATGAATTGAAAGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGTGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.115	93.627	0.544
comEA	Lactococcus lactis subsp. cremoris KW2	33.784	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.071	100	0.363