NTDB

Overview

Name	comGF	Type	Machinery gene
Locus tag	AH5611_RS07265	Genome accession	NZ_CP092055
Coordinates	1527918..1528415 (-)	Length	165 a.a.
NCBI ID	WP_001796472.1	Uniprot ID	A0A5F0HJ55
Organism	Staphylococcus aureus strain USA600
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1522918..1533415

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AH5611_RS07240 (AH5611_001404)	gcvPB	1523080..1524552 (-)	1473	WP_000202176.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
AH5611_RS07245 (AH5611_001405)	gcvPA	1524545..1525891 (-)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
AH5611_RS07250 (AH5611_001406)	gcvT	1525911..1527002 (-)	1092	WP_000093344.1	glycine cleavage system aminomethyltransferase GcvT	-
AH5611_RS07255 (AH5611_001407)	-	1527161..1527685 (-)	525	WP_001015120.1	shikimate kinase	-
AH5611_RS07260	-	1527675..1527821 (-)	147	WP_001789879.1	hypothetical protein	-
AH5611_RS07265 (AH5611_001408)	comGF	1527918..1528415 (-)	498	WP_001796472.1	competence type IV pilus minor pilin ComGF	Machinery gene
AH5611_RS07270 (AH5611_001409)	comGE	1528333..1528632 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
AH5611_RS07275 (AH5611_001410)	comGD	1528619..1529065 (-)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
AH5611_RS07280 (AH5611_001411)	comGC	1529043..1529354 (-)	312	WP_000472255.1	competence type IV pilus major pilin ComGC	Machinery gene
AH5611_RS07285 (AH5611_001412)	comGB	1529368..1530438 (-)	1071	WP_000776409.1	competence type IV pilus assembly protein ComGB	Machinery gene
AH5611_RS07290 (AH5611_001413)	comGA	1530410..1531384 (-)	975	WP_000697223.1	competence type IV pilus ATPase ComGA	Machinery gene
AH5611_RS07295 (AH5611_001414)	-	1531436..1532059 (-)	624	WP_001223009.1	MBL fold metallo-hydrolase	-
AH5611_RS07300 (AH5611_001415)	-	1532056..1532385 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
AH5611_RS07305 (AH5611_001416)	-	1532385..1533371 (-)	987	WP_000161307.1	ROK family glucokinase	-

Sequence

Protein

Download Length: 165 a.a. Molecular weight: 19169.89 Da Isoelectric Point: 10.1358

                                >NTDB_id=655469 AH5611_RS07265 WP_001796472.1 1527918..1528415(-) (comGF) [Staphylococcus aureus strain USA600]

MILSKVTNKYVPFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI

LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT

KTIYV

Nucleotide

Download Length: 498 bp

                                >NTDB_id=655469 AH5611_RS07265 WP_001796472.1 1527918..1528415(-) (comGF) [Staphylococcus aureus strain USA600]

ATGATATTAAGCAAAGTGACCAACAAATATGTGCCATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT

TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT

TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT

CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT

GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA

CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT

AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A5F0HJ55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	95.758	100	0.958

Detailed information NTG0655469