Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   L8956_RS17005 Genome accession   NZ_CP091882
Coordinates   3593709..3594785 (-) Length   358 a.a.
NCBI ID   WP_034311323.1    Uniprot ID   -
Organism   Peribacillus frigoritolerans strain JHS1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3588709..3599785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L8956_RS16965 (L8956_16965) - 3589578..3590375 (+) 798 WP_048688306.1 YqhG family protein -
  L8956_RS16970 (L8956_16970) - 3590420..3590605 (-) 186 WP_057278811.1 YqzE family protein -
  L8956_RS16975 (L8956_16975) comGG 3590720..3591106 (-) 387 WP_230136321.1 competence type IV pilus minor pilin ComGG -
  L8956_RS16980 (L8956_16980) comGF 3591099..3591578 (-) 480 WP_239858475.1 competence type IV pilus minor pilin ComGF -
  L8956_RS16985 (L8956_16985) - 3591526..3591864 (-) 339 WP_239858476.1 hypothetical protein -
  L8956_RS16990 (L8956_16990) comGD 3591851..3592294 (-) 444 WP_239858477.1 competence type IV pilus minor pilin ComGD -
  L8956_RS16995 (L8956_16995) comGC 3592302..3592613 (-) 312 WP_057278814.1 competence type IV pilus major pilin ComGC -
  L8956_RS17000 (L8956_17000) comGB 3592691..3593725 (-) 1035 WP_239858478.1 competence type IV pilus assembly protein ComGB -
  L8956_RS17005 (L8956_17005) comGA 3593709..3594785 (-) 1077 WP_034311323.1 competence type IV pilus ATPase ComGA Machinery gene
  L8956_RS17010 (L8956_17010) - 3594889..3595269 (-) 381 WP_095678573.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  L8956_RS17015 (L8956_17015) - 3595485..3596186 (+) 702 WP_034308391.1 helix-turn-helix domain-containing protein -
  L8956_RS17020 (L8956_17020) - 3596272..3596514 (+) 243 WP_034308393.1 DUF2626 domain-containing protein -
  L8956_RS17025 (L8956_17025) - 3596567..3597667 (-) 1101 WP_239858479.1 SAM-dependent methyltransferase -
  L8956_RS17030 (L8956_17030) - 3597972..3598598 (-) 627 WP_057278817.1 MBL fold metallo-hydrolase -
  L8956_RS17035 (L8956_17035) - 3598832..3599005 (+) 174 WP_034308396.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39756.39 Da        Isoelectric Point: 8.7572

>NTDB_id=654232 L8956_RS17005 WP_034311323.1 3593709..3594785(-) (comGA) [Peribacillus frigoritolerans strain JHS1]
MISIEKTAEKILTRAIVESASDIHIFFRGEGPHIQFRIDNKLVPQETLSFFEAERLIAHLKFLASMDIGEKRRPQSGAIT
INLANQVVGLRLSTLPTAHLESLVIRLIPQQNILPLEQLSLFPNTVQKLIALLKHSHGMLIFTGPTGSGKTTTLYSLLHH
AHEMINRNIITLEDPIENVSEKVLQVQINEKAGITYSVGLKAVLRHDPDVIMVGEVRDAETAKIAVRAALTGHLILTTMH
TRDAQGAISRLLEFGVSLLEVEQTLIGVTAQRLVELRCLPCKGDCALACKMTARNKRASVYELLYGKSLAEVLRIMGDEK
GKATVSYRQLKDEIGKAVAMGYVGSQEYERLVYHETKK

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=654232 L8956_RS17005 WP_034311323.1 3593709..3594785(-) (comGA) [Peribacillus frigoritolerans strain JHS1]
TTGATATCGATTGAAAAGACCGCAGAAAAAATACTAACCCGTGCCATCGTGGAATCGGCATCGGATATCCACATTTTTTT
TCGCGGAGAGGGGCCTCACATCCAATTCAGGATAGACAATAAGCTTGTTCCCCAGGAAACATTATCATTCTTTGAAGCTG
AAAGGCTGATCGCCCATTTGAAGTTCCTTGCCTCGATGGATATAGGGGAGAAAAGGAGGCCCCAGAGTGGTGCCATCACC
ATCAATTTGGCTAACCAGGTGGTCGGACTCCGCCTTTCCACTTTACCCACTGCCCATCTCGAAAGTTTGGTCATCCGCTT
AATACCCCAACAGAATATCCTTCCTCTAGAACAGTTATCCTTATTTCCAAACACCGTTCAAAAATTGATTGCACTCCTGA
AGCATTCCCATGGCATGCTCATATTTACCGGCCCGACCGGCAGTGGGAAAACCACGACACTATATTCCCTGCTTCACCAC
GCCCATGAGATGATCAATCGAAATATCATTACACTTGAAGATCCCATTGAAAATGTATCCGAAAAGGTACTGCAAGTCCA
AATCAATGAAAAGGCGGGCATTACGTATTCAGTCGGCCTAAAAGCTGTTCTGAGGCATGACCCGGACGTGATCATGGTTG
GGGAAGTCAGGGATGCAGAAACTGCCAAAATCGCAGTCCGTGCCGCATTAACCGGTCATTTGATACTAACGACCATGCAT
ACGAGGGACGCTCAGGGCGCCATCTCCAGATTACTGGAATTTGGTGTCAGCTTACTTGAGGTTGAACAGACTTTGATTGG
CGTGACAGCGCAGCGGCTGGTTGAATTGCGATGTCTTCCGTGTAAAGGGGACTGTGCTTTAGCTTGCAAAATGACTGCAA
GGAATAAAAGGGCAAGTGTATATGAATTGCTATATGGAAAAAGTCTGGCTGAAGTTCTCCGGATAATGGGAGATGAAAAA
GGAAAGGCAACGGTCAGCTACCGCCAATTAAAGGATGAAATCGGAAAAGCGGTTGCGATGGGCTATGTGGGATCTCAGGA
ATATGAACGGCTGGTCTACCATGAAACCAAAAAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.022

99.721

0.559
  pilB Haemophilus influenzae 86-028NP

38.218

97.207

0.372
  pilB Haemophilus influenzae Rd KW20

37.069

97.207

0.36