Detailed information NTG0065259

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BEST7003_RS12270
• Genome accession: NZ_AP012496
• Coordinates: 2472305..2472922 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis BEST7003
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2467305..2477922

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BEST7003_RS12245 (BEST7003_2415)	rpsT	2467607..2467873 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
BEST7003_RS12250 (BEST7003_2416)	holA	2467888..2468931 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
BEST7003_RS21830	-	2468971..2469120 (-)	150	WP_003229985.1	hypothetical protein	-
BEST7003_RS20850 (BEST7003_2417)	yqzM	2469161..2469295 (+)	135	WP_003229983.1	YqzM family protein	-
BEST7003_RS12260 (BEST7003_2418)	comEC	2469335..2471665 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BEST7003_RS12265 (BEST7003_2419)	comEB	2471669..2472238 (-)	570	WP_003229978.1	ComE operon protein 2	-
BEST7003_RS12270 (BEST7003_2420)	comEA	2472305..2472922 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
BEST7003_RS12275 (BEST7003_2421)	comER	2473006..2473827 (+)	822	WP_004398597.1	late competence protein ComER	-
BEST7003_RS12280 (BEST7003_2422)	yqeM	2473893..2474636 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
BEST7003_RS12285 (BEST7003_2423)	rsfS	2474633..2474989 (-)	357	WP_003229971.1	ribosome silencing factor	-
BEST7003_RS12290 (BEST7003_2424)	yqeK	2475007..2475567 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BEST7003_RS12295 (BEST7003_2425)	nadD	2475557..2476126 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
BEST7003_RS12300 (BEST7003_2426)	yhbY	2476138..2476428 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
BEST7003_RS12305 (BEST7003_2427)	aroE	2476422..2477264 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=65259 BEST7003_RS12270 WP_004398514.1 2472305..2472922(-) (comEA) [Bacillus subtilis BEST7003]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=65259 BEST7003_RS12270 WP_004398514.1 2472305..2472922(-) (comEA) [Bacillus subtilis BEST7003]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39