Detailed information NTG0647724

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: L1704_RS05630
• Genome accession: NZ_CP091107
• Coordinates: 1053012..1054334 (-)
• Length: 440 a.a.
• NCBI ID: WP_058221332.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain UC109
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1048012..1059334

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L1704_RS05600 (L1704_05600)	-	1049170..1050123 (+)	954	WP_023189344.1	PhoH family protein	-
L1704_RS05605 (L1704_05605)	-	1050181..1050660 (+)	480	WP_003130650.1	NUDIX hydrolase	-
L1704_RS05610 (L1704_05610)	ybeY	1050812..1051300 (+)	489	WP_058225935.1	rRNA maturation RNase YbeY	-
L1704_RS05615 (L1704_05615)	-	1051284..1051739 (+)	456	WP_003130653.1	diacylglycerol kinase	-
L1704_RS05620 (L1704_05620)	-	1051753..1052331 (+)	579	WP_003130655.1	nucleotidyltransferase family protein	-
L1704_RS05625 (L1704_05625)	comFC	1052365..1052769 (-)	405	WP_003130657.1	ComF family protein	Machinery gene
L1704_RS05630 (L1704_05630)	comFA	1053012..1054334 (-)	1323	WP_058221332.1	DEAD/DEAH box helicase	Machinery gene
L1704_RS05635 (L1704_05635)	-	1054391..1055020 (+)	630	WP_058221331.1	YigZ family protein	-
L1704_RS05640 (L1704_05640)	-	1055154..1055651 (+)	498	WP_003130662.1	VanZ family protein	-
L1704_RS05645 (L1704_05645)	-	1055699..1056718 (+)	1020	WP_003130663.1	DUF475 domain-containing protein	-
L1704_RS05650 (L1704_05650)	-	1056834..1057580 (+)	747	WP_003130666.1	bifunctional metallophosphatase/5'-nucleotidase	-
L1704_RS05655 (L1704_05655)	glyQ	1057865..1058818 (+)	954	WP_003130667.1	glycine--tRNA ligase subunit alpha	-

Sequence

Protein

• Length: 440 a.a.
• Molecular weight: 50201.71 Da
• Isoelectric Point: 10.1065

>NTDB_id=647724 L1704_RS05630 WP_058221332.1 1053012..1054334(-) (comFA) [Lactococcus lactis subsp. lactis strain UC109]
MSTNQEKLFGRLLLKNDILQLIKNTDKISVSKIFSNFLLETKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYV
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide

• Length: 1323 bp

>NTDB_id=647724 L1704_RS05630 WP_058221332.1 1053012..1054334(-) (comFA) [Lactococcus lactis subsp. lactis strain UC109]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTGCAACTTATAAAAAATACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAACGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTTTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATGTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACATCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (114-233)

• Helicase conserved C-terminal domain (Helicase_C) (300-403)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	74.5	90.909	0.677
comFA/cflA	Streptococcus mitis SK321	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae Rx1	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae D39	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae R6	52.01	90.455	0.47
comFA/cflA	Streptococcus pneumoniae TIGR4	52.01	90.455	0.47
comFA/cflA	Streptococcus mitis NCTC 12261	51.256	90.455	0.464
comFA	Bacillus subtilis subsp. subtilis str. 168	38.018	98.636	0.375