Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LL229_RS07810 Genome accession   NZ_CP090823
Coordinates   1515142..1516464 (+) Length   440 a.a.
NCBI ID   WP_021214546.1    Uniprot ID   A0A2X0PIL8
Organism   Lactococcus lactis subsp. lactis strain 229     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1500651..1560603 1515142..1516464 within 0


Gene organization within MGE regions


Location: 1500651..1560603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL229_RS07745 (LL229_1234) - 1502041..1502742 (-) 702 WP_010905758.1 ABC transporter ATP-binding protein -
  LL229_RS07750 (LL229_1233) nadE 1502870..1503694 (-) 825 WP_004255352.1 ammonia-dependent NAD(+) synthetase -
  LL229_RS07755 (LL229_1232) - 1503755..1504243 (-) 489 WP_010905757.1 GNAT family N-acetyltransferase -
  LL229_RS07760 (LL229_1231) - 1504240..1505712 (-) 1473 WP_031296478.1 nicotinate phosphoribosyltransferase -
  LL229_RS07765 (LL229_1229) - 1506252..1506839 (+) 588 WP_010905755.1 nicotinate-nucleotide adenylyltransferase -
  LL229_RS07770 (LL229_1228) - 1506862..1508073 (-) 1212 WP_010905754.1 voltage-gated chloride channel family protein -
  LL229_RS07775 (LL229_1227) - 1508284..1508526 (-) 243 WP_004255340.1 DUF896 domain-containing protein -
  LL229_RS07780 (LL229_1226) glyS 1508637..1510658 (-) 2022 WP_003130669.1 glycine--tRNA ligase subunit beta -
  LL229_RS07785 (LL229_1225) glyQ 1510658..1511611 (-) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -
  LL229_RS07790 (LL229_1224) - 1511896..1512642 (-) 747 WP_003130666.1 bifunctional metallophosphatase/5'-nucleotidase -
  LL229_RS07795 (LL229_1223) - 1512758..1513777 (-) 1020 WP_003130663.1 DUF475 domain-containing protein -
  LL229_RS07800 (LL229_1222) - 1513825..1514322 (-) 498 WP_003130662.1 VanZ family protein -
  LL229_RS07805 (LL229_1221) - 1514456..1515085 (-) 630 WP_021214545.1 YigZ family protein -
  LL229_RS07810 (LL229_1220) comFA 1515142..1516464 (+) 1323 WP_021214546.1 DEAD/DEAH box helicase Machinery gene
  LL229_RS07815 (LL229_03075) comFC 1516707..1517111 (+) 405 WP_003130657.1 ComF family protein Machinery gene
  LL229_RS07820 (LL229_1218) - 1517145..1517723 (-) 579 WP_003130655.1 nucleotidyltransferase family protein -
  LL229_RS07825 (LL229_1217) - 1517737..1518192 (-) 456 WP_003130653.1 diacylglycerol kinase family protein -
  LL229_RS07830 (LL229_1216) ybeY 1518176..1518664 (-) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  LL229_RS07835 (LL229_1215) - 1518816..1519295 (-) 480 WP_003130650.1 NUDIX hydrolase -
  LL229_RS07840 (LL229_1214) - 1519353..1520321 (-) 969 WP_003130649.1 PhoH family protein -
  LL229_RS07845 (LL229_1212) - 1520423..1521611 (-) 1189 Protein_1489 Nramp family divalent metal transporter -
  LL229_RS07850 (LL229_1211) rpmA 1521887..1522171 (-) 285 WP_003130646.1 50S ribosomal protein L27 -
  LL229_RS07855 (LL229_1210) - 1522173..1522508 (-) 336 WP_003130645.1 ribosomal-processing cysteine protease Prp -
  LL229_RS07860 (LL229_1209) rplU 1522511..1522825 (-) 315 WP_010905746.1 50S ribosomal protein L21 -
  LL229_RS07865 (LL229_1208) - 1522995..1524170 (-) 1176 WP_010905745.1 DUF998 domain-containing protein -
  LL229_RS07870 (LL229_1207) - 1524163..1524564 (-) 402 WP_031296481.1 DUF1934 domain-containing protein -
  LL229_RS07875 (LL229_1206) - 1524739..1526100 (+) 1362 WP_003130634.1 HD domain-containing protein -
  LL229_RS07880 (LL229_1205) yidA 1526147..1526962 (+) 816 WP_003130632.1 sugar-phosphatase -
  LL229_RS07885 (LL229_1204) - 1527084..1527449 (+) 366 WP_010905743.1 iron-sulfur cluster biosynthesis family protein -
  LL229_RS07890 (LL229_1203) - 1527488..1528261 (-) 774 WP_010905742.1 Nif3-like dinuclear metal center hexameric protein -
  LL229_RS07895 (LL229_1202) - 1528258..1528950 (-) 693 WP_003130628.1 tRNA (adenine(22)-N(1))-methyltransferase TrmK -
  LL229_RS07900 (LL229_1201) nth 1528943..1529599 (-) 657 WP_003130627.1 endonuclease III -
  LL229_RS07905 (LL229_1200) - 1529621..1530289 (-) 669 WP_010905741.1 DnaD domain-containing protein -
  LL229_RS07910 (LL229_1198) - 1530696..1531967 (-) 1272 WP_031296484.1 dihydroorotase -
  LL229_RS07915 (LL229_1197) pyrE 1532122..1532751 (-) 630 WP_003132758.1 orotate phosphoribosyltransferase -
  LL229_RS07920 (LL229_03080) - 1532732..1532860 (+) 129 WP_003132757.1 hypothetical protein -
  LL229_RS07925 (LL229_1195) - 1533333..1533494 (-) 162 WP_021214547.1 hypothetical protein -
  LL229_RS07930 (LL229_1194) - 1533559..1533747 (-) 189 WP_017864422.1 hypothetical protein -
  LL229_RS07935 (LL229_1193) - 1533781..1533963 (-) 183 WP_003132753.1 hypothetical protein -
  LL229_RS07940 (LL229_1192) - 1533973..1534704 (-) 732 WP_003132752.1 hypothetical protein -
  LL229_RS07945 (LL229_1191) - 1534951..1535808 (-) 858 WP_010905739.1 XRE/MutR family transcriptional regulator -
  LL229_RS07950 (LL229_1190) - 1535983..1536168 (-) 186 WP_003132749.1 hypothetical protein -
  LL229_RS07955 (LL229_1189) - 1536684..1537049 (-) 366 WP_003132744.1 hypothetical protein -
  LL229_RS07960 (LL229_1188) - 1537051..1537851 (-) 801 WP_010905736.1 hypothetical protein -
  LL229_RS07965 (LL229_1187) - 1538040..1539947 (-) 1908 WP_021214548.1 ABC-F family ATP-binding cassette domain-containing protein -
  LL229_RS07970 (LL229_1186) - 1540125..1540517 (-) 393 WP_003132739.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LL229_RS07975 (LL229_1185) - 1540742..1541392 (+) 651 WP_023349143.1 redox-sensing transcriptional repressor Rex -
  LL229_RS07980 (LL229_1184) - 1541894..1542673 (-) 780 WP_010905733.1 peptidoglycan amidohydrolase family protein -
  LL229_RS07985 (LL229_1183) - 1542673..1542960 (-) 288 WP_010905732.1 phage holin -
  LL229_RS07990 (LL229_1182) - 1542973..1543197 (-) 225 WP_010905731.1 hemolysin XhlA family protein -
  LL229_RS07995 (LL229_1181) - 1543223..1543450 (-) 228 WP_010905730.1 hypothetical protein -
  LL229_RS08000 (LL229_1180) - 1543452..1543790 (-) 339 WP_010905729.1 hypothetical protein -
  LL229_RS08005 (LL229_1178) - 1544243..1545073 (-) 831 WP_010905727.1 hypothetical protein -
  LL229_RS08010 (LL229_1177) - 1545066..1546667 (-) 1602 WP_010905726.1 DUF2479 domain-containing protein -
  LL229_RS08015 (LL229_1176) - 1546680..1549364 (-) 2685 WP_023349144.1 phage tail spike protein -
  LL229_RS08020 (LL229_1175) - 1549361..1550059 (-) 699 WP_227028649.1 phage tail protein -
  LL229_RS08025 (LL229_1174) - 1550111..1552372 (-) 2262 WP_010905723.1 phage tail tape measure protein -
  LL229_RS08030 (LL229_03085) - 1552386..1552562 (-) 177 WP_010905722.1 hypothetical protein -
  LL229_RS08035 (LL229_1173) - 1552589..1552948 (-) 360 WP_010905721.1 hypothetical protein -
  LL229_RS08040 (LL229_1172) - 1552958..1553566 (-) 609 WP_010905720.1 major tail protein -
  LL229_RS08045 (LL229_1171) - 1553584..1553901 (-) 318 WP_010905719.1 hypothetical protein -
  LL229_RS08050 (LL229_1170) - 1553898..1554269 (-) 372 WP_010905718.1 HK97 gp10 family phage protein -
  LL229_RS08055 (LL229_1169) - 1554259..1554576 (-) 318 WP_010905717.1 phage head closure protein -
  LL229_RS08060 (LL229_1168) - 1554563..1554844 (-) 282 WP_010905716.1 hypothetical protein -
  LL229_RS08065 (LL229_1167) - 1554837..1555694 (-) 858 WP_010905715.1 hypothetical protein -
  LL229_RS08070 (LL229_1166) - 1555749..1556942 (-) 1194 WP_010905714.1 phage major capsid protein -
  LL229_RS08075 (LL229_1165) - 1556914..1557510 (-) 597 WP_010905713.1 HK97 family phage prohead protease -
  LL229_RS08080 (LL229_1164) - 1557525..1558730 (-) 1206 WP_010905712.1 phage portal protein -
  LL229_RS08085 (LL229_1163) - 1558744..1560513 (-) 1770 WP_032941531.1 terminase large subunit -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50187.69 Da        Isoelectric Point: 10.1065

>NTDB_id=644957 LL229_RS07810 WP_021214546.1 1515142..1516464(+) (comFA) [Lactococcus lactis subsp. lactis strain 229]
MSTNQEKLFGRLLLKNDILQLIKNTDKISVSKIFSNFLLETKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDDFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYV
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=644957 LL229_RS07810 WP_021214546.1 1515142..1516464(+) (comFA) [Lactococcus lactis subsp. lactis strain 229]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTGCAACTTATAAAAAATACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAACGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGATTTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTTTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATGTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACATCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(114-233)

Helicase conserved C-terminal domain (Helicase_C)

(300-403)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2X0PIL8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.25

90.909

0.675
  comFA/cflA Streptococcus mitis SK321

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae Rx1

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae D39

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae R6

52.01

90.455

0.47
  comFA/cflA Streptococcus pneumoniae TIGR4

52.01

90.455

0.47
  comFA/cflA Streptococcus mitis NCTC 12261

51.256

90.455

0.464
  comFA Bacillus subtilis subsp. subtilis str. 168

38.018

98.636

0.375