Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   LZI70_RS08265 Genome accession   NZ_CP090614
Coordinates   1877675..1878268 (+) Length   197 a.a.
NCBI ID   WP_255229757.1    Uniprot ID   -
Organism   Vibrio pelagius strain WXL662     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1872675..1883268
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LZI70_RS08250 (LZI70_08240) - 1873368..1875926 (-) 2559 WP_255229752.1 penicillin-binding protein 1A -
  LZI70_RS08255 (LZI70_08245) pilM 1876074..1877099 (+) 1026 WP_255229753.1 type IV pilus assembly protein PilM Machinery gene
  LZI70_RS08260 (LZI70_08250) pilN 1877083..1877682 (+) 600 WP_255229755.1 PilN domain-containing protein Machinery gene
  LZI70_RS08265 (LZI70_08255) pilO 1877675..1878268 (+) 594 WP_255229757.1 type 4a pilus biogenesis protein PilO Machinery gene
  LZI70_RS08270 (LZI70_08260) pilP 1878255..1878773 (+) 519 WP_255229759.1 pilus assembly protein PilP Machinery gene
  LZI70_RS08275 (LZI70_08265) - 1878914..1880530 (+) 1617 Protein_1530 type IV pilus secretin PilQ -
  LZI70_RS08280 (LZI70_08270) aroK 1880705..1881223 (+) 519 WP_032550005.1 shikimate kinase AroK -
  LZI70_RS08285 (LZI70_08275) aroB 1881248..1882336 (+) 1089 WP_255229760.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 22875.21 Da        Isoelectric Point: 4.5763

>NTDB_id=643996 LZI70_RS08265 WP_255229757.1 1877675..1878268(+) (pilO) [Vibrio pelagius strain WXL662]
MVNLQDLDVDEITEWPLLPQLLVIALLMLIVQGFGTWFYVLPLNDELDQMKQQEQTLKTTLRIKANKVATLPALQNQLDE
LTSRYDYLLEQLPVQKELASMLASVNELGLDNHLTFTRIDWGEKQNKAFLYRLPLNIELTGDYHDIGEFSAAIAKLPRII
SFDDVTWQRVSQESSTLHFRVRAYTYQFKAEVKDESR

Nucleotide


Download         Length: 594 bp        

>NTDB_id=643996 LZI70_RS08265 WP_255229757.1 1877675..1878268(+) (pilO) [Vibrio pelagius strain WXL662]
ATGGTTAATTTGCAAGACCTCGATGTTGATGAAATTACGGAATGGCCGTTGCTGCCTCAGTTGTTGGTGATTGCTTTACT
GATGTTGATCGTTCAAGGCTTTGGGACTTGGTTTTATGTGCTGCCACTCAATGATGAGTTGGATCAGATGAAGCAGCAAG
AGCAGACTTTAAAGACCACCCTTCGTATTAAAGCCAATAAAGTGGCGACTTTGCCCGCGCTTCAGAATCAACTCGATGAA
TTGACCAGTCGCTACGACTACTTGCTTGAACAACTGCCAGTTCAAAAAGAGTTGGCGAGCATGCTGGCCTCTGTGAATGA
GCTCGGTTTAGACAATCATCTCACTTTTACTCGCATTGACTGGGGGGAAAAACAGAACAAGGCGTTTCTGTATCGATTGC
CTTTGAATATTGAACTCACGGGTGACTACCACGATATCGGTGAATTTTCTGCGGCGATTGCCAAGCTGCCTCGCATCATA
AGCTTTGATGACGTGACCTGGCAAAGAGTCAGCCAAGAGAGCAGTACTCTGCATTTTCGTGTTCGCGCATACACCTATCA
ATTTAAGGCGGAGGTGAAAGATGAATCTCGCTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

75.385

98.985

0.746
  pilO Vibrio campbellii strain DS40M4

72.487

95.939

0.695