Detailed information NTG0642988

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OHL99_RS10285
• Genome accession: NZ_CP107270
• Coordinates: 1947413..1948639 (-)
• Length: 408 a.a.
• NCBI ID: WP_003697786.1
• Uniprot ID: B4RPS5
• Organism: Neisseria gonorrhoeae strain 1081168
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1942413..1953639

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OHL99_RS10275 (OHL99_10275)	-	1944378..1944662 (+)	285	WP_003692093.1	GIY-YIG nuclease family protein	-
OHL99_RS10280 (OHL99_10280)	yccS	1945146..1947296 (-)	2151	WP_003697785.1	YccS family putative transporter	-
OHL99_RS10285 (OHL99_10285)	pilU	1947413..1948639 (-)	1227	WP_003697786.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OHL99_RS10290 (OHL99_10290)	pilT	1948803..1949846 (-)	1044	WP_003690200.1	type IV pilus twitching motility protein PilT	Machinery gene
OHL99_RS10295 (OHL99_10295)	-	1949966..1950652 (+)	687	WP_003690201.1	YggS family pyridoxal phosphate-dependent enzyme	-
OHL99_RS10300 (OHL99_10300)	-	1950652..1951059 (+)	408	WP_003690202.1	hypothetical protein	-
OHL99_RS10305 (OHL99_10305)	proC	1951108..1951899 (+)	792	WP_003694504.1	pyrroline-5-carboxylate reductase	-
OHL99_RS10310 (OHL99_10310)	dksA	1952048..1952464 (+)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45668.30 Da
• Isoelectric Point: 7.2909

>NTDB_id=642988 OHL99_RS10285 WP_003697786.1 1947413..1948639(-) (pilU) [Neisseria gonorrhoeae strain 1081168]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=642988 OHL99_RS10285 WP_003697786.1 1947413..1948639(-) (pilU) [Neisseria gonorrhoeae strain 1081168]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCGGGAATTGGGCTTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: B4RPS5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.875	88.48	0.397
pilU	Pseudomonas stutzeri DSM 10701	45.225	87.255	0.395
pilU	Vibrio cholerae strain A1552	47.024	82.353	0.387