Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   IHC87_RS01960 Genome accession   NZ_CP090501
Coordinates   395287..395673 (-) Length   128 a.a.
NCBI ID   WP_237033351.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain CSP DAM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 390287..400673
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHC87_RS01940 (IHC87_01940) coaE 390639..391241 (-) 603 WP_237033347.1 dephospho-CoA kinase -
  IHC87_RS01945 (IHC87_01945) pilD 391243..392121 (-) 879 WP_237033348.1 prepilin peptidase Machinery gene
  IHC87_RS01950 (IHC87_01950) pilC 392303..393529 (-) 1227 WP_237033349.1 type II secretion system F family protein Machinery gene
  IHC87_RS01955 (IHC87_01955) pilB 393595..395286 (-) 1692 WP_237033350.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IHC87_RS01960 (IHC87_01960) pilA 395287..395673 (-) 387 WP_237033351.1 pilin Machinery gene
  IHC87_RS01965 (IHC87_01965) ampD 396068..396646 (+) 579 WP_069531889.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  IHC87_RS01970 (IHC87_01970) pdhR 397006..397779 (+) 774 WP_005297609.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  IHC87_RS01975 (IHC87_01975) aceE 397860..400520 (+) 2661 WP_065171770.1 pyruvate dehydrogenase (acetyl-transferring), homodimeric type -

Sequence

Protein


Download         Length: 128 a.a.        Molecular weight: 12844.66 Da        Isoelectric Point: 7.1311

>NTDB_id=642970 IHC87_RS01960 WP_237033351.1 395287..395673(-) (pilA) [Photobacterium damselae subsp. damselae strain CSP DAM1]
MKGQKGFTLIELMIVVAVIGVLSAIAVPKYQEYVKKGALGAALASVTAHKTNVEDSIATLGTFPSVSAAFGIGSISSPGS
SDAVGTITATIDEGAANNATVTISRDANGVWTCTHSLSSTIKISGCQG

Nucleotide


Download         Length: 387 bp        

>NTDB_id=642970 IHC87_RS01960 WP_237033351.1 395287..395673(-) (pilA) [Photobacterium damselae subsp. damselae strain CSP DAM1]
ATGAAAGGACAAAAGGGTTTTACCTTAATTGAATTGATGATCGTAGTGGCGGTTATTGGTGTGTTATCAGCAATTGCCGT
ACCAAAATATCAAGAATATGTAAAAAAAGGAGCATTAGGAGCTGCATTAGCTTCAGTAACTGCTCACAAAACTAATGTAG
AAGACTCAATTGCAACTTTAGGTACATTTCCTTCTGTTAGTGCCGCATTTGGGATAGGTAGTATTTCATCTCCTGGCTCA
TCAGACGCCGTTGGTACAATTACAGCAACAATAGATGAAGGTGCAGCCAATAATGCAACAGTAACTATCTCAAGAGATGC
AAATGGAGTTTGGACATGTACACACTCTCTTAGCTCAACAATAAAGATCAGTGGTTGTCAAGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-122)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

51.429

100

0.563
  pilA Vibrio cholerae strain A1552

51.429

100

0.563
  pilA Vibrio cholerae C6706

51.429

100

0.563
  pilA Pseudomonas aeruginosa PAK

40.541

100

0.469
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.045

100

0.43
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.85

100

0.414
  pilA Vibrio parahaemolyticus RIMD 2210633

38.686

100

0.414
  comP Acinetobacter baylyi ADP1

34.694

100

0.398
  pilA2 Legionella pneumophila str. Paris

37.594

100

0.391
  pilA/pilA1 Eikenella corrodens VA1

34.752

100

0.383
  pilA2 Legionella pneumophila strain ERS1305867

36.842

100

0.383
  pilA Acinetobacter baumannii strain A118

35.766

100

0.383