Detailed information NTG0642848

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NDN57_RS10175
• Genome accession: NZ_CP107264
• Coordinates: 1943587..1944813 (+)
• Length: 408 a.a.
• NCBI ID: WP_003692100.1
• Uniprot ID: Q5F5L4
• Organism: Neisseria gonorrhoeae strain 10231
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1938587..1949813

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NDN57_RS10150 (NDN57_010150)	dksA	1939762..1940178 (-)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-
NDN57_RS10155 (NDN57_010155)	proC	1940327..1941118 (-)	792	WP_003694504.1	pyrroline-5-carboxylate reductase	-
NDN57_RS10160 (NDN57_010160)	-	1941167..1941574 (-)	408	WP_003690202.1	hypothetical protein	-
NDN57_RS10165 (NDN57_010165)	-	1941574..1942260 (-)	687	WP_003692103.1	YggS family pyridoxal phosphate-dependent enzyme	-
NDN57_RS10170 (NDN57_010170)	pilT	1942380..1943423 (+)	1044	WP_003690200.1	type IV pilus twitching motility protein PilT	Machinery gene
NDN57_RS10175 (NDN57_010175)	pilU	1943587..1944813 (+)	1227	WP_003692100.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NDN57_RS10180 (NDN57_010180)	yccS	1944930..1947080 (+)	2151	WP_003692098.1	YccS family putative transporter	-
NDN57_RS10185 (NDN57_010185)	-	1947563..1947847 (-)	285	WP_003692093.1	GIY-YIG nuclease family protein	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45698.33 Da
• Isoelectric Point: 7.2909

>NTDB_id=642848 NDN57_RS10175 WP_003692100.1 1943587..1944813(+) (pilU) [Neisseria gonorrhoeae strain 10231]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGTHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=642848 NDN57_RS10175 WP_003692100.1 1943587..1944813(+) (pilU) [Neisseria gonorrhoeae strain 10231]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCACACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCGGGAATTGGGCTTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAGGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: Q5F5L4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.875	88.48	0.397
pilU	Pseudomonas stutzeri DSM 10701	45.225	87.255	0.395
pilU	Vibrio cholerae strain A1552	47.024	82.353	0.387