Detailed information NTG0642713

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LXM91_RS12520
• Genome accession: NZ_CP090477
• Coordinates: 2557830..2558444 (-)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain W0101
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2552830..2563444

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LXM91_RS12490 (LXM91_12490)	rpsT	2553133..2553399 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
LXM91_RS12495 (LXM91_12495)	holA	2553416..2554457 (-)	1042	Protein_2419	DNA polymerase III subunit delta	-
LXM91_RS12500 (LXM91_12500)	-	2554497..2554648 (-)	152	Protein_2420	hypothetical protein	-
LXM91_RS12505 (LXM91_12505)	-	2554689..2554823 (+)	135	WP_003152870.1	YqzM family protein	-
LXM91_RS12510 (LXM91_12510)	comEC	2554842..2557193 (-)	2352	WP_039254415.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LXM91_RS12515 (LXM91_12515)	-	2557194..2557763 (-)	570	WP_003152868.1	ComE operon protein 2	-
LXM91_RS12520 (LXM91_12520)	comEA	2557830..2558444 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
LXM91_RS12525 (LXM91_12525)	comER	2558503..2559324 (+)	822	WP_039254409.1	late competence protein ComER	-
LXM91_RS12530 (LXM91_12530)	-	2559393..2560130 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
LXM91_RS12535 (LXM91_12535)	rsfS	2560127..2560483 (-)	357	WP_007408260.1	ribosome silencing factor	-
LXM91_RS12540 (LXM91_12540)	yqeK	2560501..2561061 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LXM91_RS12545 (LXM91_12545)	-	2561051..2561620 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
LXM91_RS12550 (LXM91_12550)	yhbY	2561631..2561921 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
LXM91_RS12555 (LXM91_12555)	aroE	2561915..2562757 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=642713 LXM91_RS12520 WP_015240245.1 2557830..2558444(-) (comEA) [Bacillus amyloliquefaciens strain W0101]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=642713 LXM91_RS12520 WP_015240245.1 2557830..2558444(-) (comEA) [Bacillus amyloliquefaciens strain W0101]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363