NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	LXM91_RS12095	Genome accession	NZ_CP090477
Coordinates	2481321..2482391 (-)	Length	356 a.a.
NCBI ID	WP_003153083.1	Uniprot ID	-
Organism	Bacillus amyloliquefaciens strain W0101
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2476321..2487391

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LXM91_RS12045 (LXM91_12045)	-	2476381..2476965 (-)	585	WP_015240205.1	signal peptidase I	-
LXM91_RS12050 (LXM91_12050)	tapA	2476937..2477608 (-)	672	WP_031378945.1	amyloid fiber anchoring/assembly protein TapA	-
LXM91_RS12055 (LXM91_12055)	-	2477867..2478196 (+)	330	WP_039254490.1	DUF3889 domain-containing protein	-
LXM91_RS12060 (LXM91_12060)	-	2478236..2478415 (-)	180	WP_003153093.1	YqzE family protein	-
LXM91_RS12065 (LXM91_12065)	comGG	2478472..2478849 (-)	378	WP_015417814.1	competence type IV pilus minor pilin ComGG	Machinery gene
LXM91_RS12070 (LXM91_12070)	comGF	2478850..2479350 (-)	501	WP_257738552.1	competence type IV pilus minor pilin ComGF	-
LXM91_RS12075 (LXM91_12075)	comGE	2479259..2479573 (-)	315	WP_012117982.1	competence type IV pilus minor pilin ComGE	-
LXM91_RS12080 (LXM91_12080)	comGD	2479557..2479994 (-)	438	WP_015417817.1	competence type IV pilus minor pilin ComGD	Machinery gene
LXM91_RS12085 (LXM91_12085)	comGC	2479984..2480292 (-)	309	WP_015417818.1	competence type IV pilus major pilin ComGC	Machinery gene
LXM91_RS12090 (LXM91_12090)	comGB	2480297..2481334 (-)	1038	WP_015417819.1	competence type IV pilus assembly protein ComGB	Machinery gene
LXM91_RS12095 (LXM91_12095)	comGA	2481321..2482391 (-)	1071	WP_003153083.1	competence type IV pilus ATPase ComGA	Machinery gene
LXM91_RS12100 (LXM91_12100)	-	2482584..2483534 (-)	951	WP_015417820.1	magnesium transporter CorA family protein	-
LXM91_RS12105 (LXM91_12105)	-	2483680..2484981 (+)	1302	WP_012117986.1	hemolysin family protein	-
LXM91_RS12110 (LXM91_12110)	-	2485019..2485849 (-)	831	WP_039254482.1	STAS domain-containing protein	-
LXM91_RS12120 (LXM91_12120)	-	2486056..2486436 (-)	381	WP_003153079.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
LXM91_RS12125 (LXM91_12125)	-	2486673..2486918 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40529.85 Da Isoelectric Point: 9.4729

                                >NTDB_id=642709 LXM91_RS12095 WP_003153083.1 2481321..2482391(-) (comGA) [Bacillus amyloliquefaciens strain W0101]

MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=642709 LXM91_RS12095 WP_003153083.1 2481321..2482391(-) (comGA) [Bacillus amyloliquefaciens strain W0101]

GTGAAAAATATTGAAACATATAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC

GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG

TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT

CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA

AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT

GCAAAAAGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATTTTAG

GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT

ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC

TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA

GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG

CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA

CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.022	100	0.82
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0642709