NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	LXH20_RS11920	Genome accession	NZ_CP090150
Coordinates	2474753..2475823 (-)	Length	356 a.a.
NCBI ID	WP_014418378.1	Uniprot ID	A0AAP4DGV9
Organism	Bacillus velezensis strain BR-01
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2469753..2480823

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LXH20_RS11870 (LXH20_11870)	sipW	2469812..2470396 (-)	585	WP_022552967.1	signal peptidase I SipW	-
LXH20_RS11875 (LXH20_11875)	tapA	2470368..2471039 (-)	672	WP_014418371.1	amyloid fiber anchoring/assembly protein TapA	-
LXH20_RS11880 (LXH20_11880)	-	2471298..2471627 (+)	330	WP_012117979.1	DUF3889 domain-containing protein	-
LXH20_RS11885 (LXH20_11885)	-	2471668..2471847 (-)	180	WP_022552966.1	YqzE family protein	-
LXH20_RS11890 (LXH20_11890)	comGG	2471904..2472281 (-)	378	WP_022552965.1	competence type IV pilus minor pilin ComGG	Machinery gene
LXH20_RS11895 (LXH20_11895)	comGF	2472282..2472677 (-)	396	WP_046559876.1	competence type IV pilus minor pilin ComGF	-
LXH20_RS11900 (LXH20_11900)	comGE	2472691..2473005 (-)	315	WP_021494312.1	competence type IV pilus minor pilin ComGE	Machinery gene
LXH20_RS11905 (LXH20_11905)	comGD	2472989..2473426 (-)	438	WP_007612572.1	competence type IV pilus minor pilin ComGD	Machinery gene
LXH20_RS11910 (LXH20_11910)	comGC	2473416..2473724 (-)	309	WP_014418376.1	competence type IV pilus major pilin ComGC	Machinery gene
LXH20_RS11915 (LXH20_11915)	comGB	2473729..2474766 (-)	1038	WP_022552962.1	competence type IV pilus assembly protein ComGB	Machinery gene
LXH20_RS11920 (LXH20_11920)	comGA	2474753..2475823 (-)	1071	WP_014418378.1	competence type IV pilus ATPase ComGA	Machinery gene
LXH20_RS11925 (LXH20_11925)	-	2476016..2476966 (-)	951	WP_014418379.1	magnesium transporter CorA family protein	-
LXH20_RS11930 (LXH20_11930)	-	2477112..2478413 (+)	1302	WP_022552961.1	hemolysin family protein	-
LXH20_RS11935 (LXH20_11935)	-	2478451..2479281 (-)	831	WP_022552960.1	STAS domain-containing protein	-
LXH20_RS11945 (LXH20_11945)	-	2479488..2479868 (-)	381	WP_003153079.1	Spx/MgsR family RNA polymerase-binding regulatory protein	-
LXH20_RS11950 (LXH20_11950)	-	2480105..2480350 (+)	246	WP_003153078.1	DUF2626 domain-containing protein	-

Sequence

Protein

Download Length: 356 a.a. Molecular weight: 40529.81 Da Isoelectric Point: 9.3485

                                >NTDB_id=640906 LXH20_RS11920 WP_014418378.1 2474753..2475823(-) (comGA) [Bacillus velezensis strain BR-01]

MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS

LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY

AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH

TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE

HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide

Download Length: 1071 bp

                                >NTDB_id=640906 LXH20_RS11920 WP_014418378.1 2474753..2475823(-) (comGA) [Bacillus velezensis strain BR-01]

GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC

GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG

TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT

CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT

TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA

AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT

GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA

GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG

GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCAATGACGGGACACCTCGTTCTTTCAAGCCTGCAT

ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC

CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA

GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG

CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA

CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Bacillus subtilis subsp. subtilis str. 168	82.303	100	0.823
pilB	Glaesserella parasuis strain SC1401	38.793	97.753	0.379
pilF	Thermus thermophilus HB27	37.892	98.596	0.374

Detailed information NTG0640906