Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   LXE94_RS12915 Genome accession   NZ_CP090125
Coordinates   2419231..2419848 (-) Length   205 a.a.
NCBI ID   WP_257986083.1    Uniprot ID   -
Organism   Bacillus subtilis strain Bs96     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2414231..2424848
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LXE94_RS12885 (LXE94_12860) rpsT 2414533..2414799 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  LXE94_RS12890 (LXE94_12865) holA 2414814..2415857 (-) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  LXE94_RS12895 (LXE94_12870) - 2415897..2416046 (-) 150 WP_003229985.1 hypothetical protein -
  LXE94_RS12900 (LXE94_12875) yqzM 2416087..2416221 (+) 135 WP_003229983.1 YqzM family protein -
  LXE94_RS12905 (LXE94_12880) comEC 2416261..2418591 (-) 2331 WP_257986082.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LXE94_RS12910 (LXE94_12885) comEB 2418595..2419164 (-) 570 WP_003229978.1 ComE operon protein 2 -
  LXE94_RS12915 (LXE94_12890) comEA 2419231..2419848 (-) 618 WP_257986083.1 competence protein ComEA Machinery gene
  LXE94_RS12920 (LXE94_12895) comER 2419932..2420753 (+) 822 WP_134981848.1 late competence protein ComER -
  LXE94_RS12925 (LXE94_12900) yqeM 2420819..2421562 (-) 744 WP_151870165.1 class I SAM-dependent methyltransferase -
  LXE94_RS12930 (LXE94_12905) rsfS 2421559..2421915 (-) 357 WP_014480315.1 ribosome silencing factor -
  LXE94_RS12935 (LXE94_12910) yqeK 2421933..2422493 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  LXE94_RS12940 (LXE94_12915) nadD 2422483..2423052 (-) 570 WP_041333756.1 nicotinate-nucleotide adenylyltransferase -
  LXE94_RS12945 (LXE94_12920) yhbY 2423064..2423354 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  LXE94_RS12950 (LXE94_12925) aroE 2423348..2424190 (-) 843 WP_257986084.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21721.50 Da        Isoelectric Point: 4.8157

>NTDB_id=640710 LXE94_RS12915 WP_257986083.1 2419231..2419848(-) (comEA) [Bacillus subtilis strain Bs96]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESNGTIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGIVVYIPKKGEETAVQQGGGVSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=640710 LXE94_RS12915 WP_257986083.1 2419231..2419848(-) (comEA) [Bacillus subtilis strain Bs96]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCAATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGGAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGATAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGTGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATATCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.049

100

0.98
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39