Detailed information NTG0638658

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NDL74_RS10255
• Genome accession: NZ_CP106921
• Coordinates: 1951429..1952655 (+)
• Length: 408 a.a.
• NCBI ID: WP_003692100.1
• Uniprot ID: Q5F5L4
• Organism: Neisseria gonorrhoeae strain 10272
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1946429..1957655

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NDL74_RS10225 (NDL74_10225)	-	1947461..1947589 (-)	129	WP_256204576.1	hypothetical protein	-
NDL74_RS10230 (NDL74_10230)	dksA	1947604..1948020 (-)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-
NDL74_RS10235 (NDL74_10235)	proC	1948169..1948960 (-)	792	WP_003694504.1	pyrroline-5-carboxylate reductase	-
NDL74_RS10240 (NDL74_10240)	-	1949009..1949416 (-)	408	WP_003690202.1	hypothetical protein	-
NDL74_RS10245 (NDL74_10245)	-	1949416..1950102 (-)	687	WP_003692103.1	YggS family pyridoxal phosphate-dependent enzyme	-
NDL74_RS10250 (NDL74_10250)	pilT	1950222..1951265 (+)	1044	WP_003690200.1	type IV pilus twitching motility protein PilT	Machinery gene
NDL74_RS10255 (NDL74_10255)	pilU	1951429..1952655 (+)	1227	WP_003692100.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NDL74_RS10260 (NDL74_10260)	yccS	1952772..1954922 (+)	2151	WP_003692098.1	YccS family putative transporter	-
NDL74_RS10265 (NDL74_10265)	-	1955404..1955688 (-)	285	WP_003692093.1	GIY-YIG nuclease family protein	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45698.33 Da
• Isoelectric Point: 7.2909

>NTDB_id=638658 NDL74_RS10255 WP_003692100.1 1951429..1952655(+) (pilU) [Neisseria gonorrhoeae strain 10272]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGTHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=638658 NDL74_RS10255 WP_003692100.1 1951429..1952655(+) (pilU) [Neisseria gonorrhoeae strain 10272]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCACACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCGGGAATTGGGCTTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAGGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: Q5F5L4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.875	88.48	0.397
pilU	Pseudomonas stutzeri DSM 10701	45.225	87.255	0.395
pilU	Vibrio cholerae strain A1552	47.024	82.353	0.387