Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   LUM42_RS14460 Genome accession   NZ_CP089603
Coordinates   3094322..3094912 (-) Length   196 a.a.
NCBI ID   WP_232658224.1    Uniprot ID   -
Organism   Vibrio furnissii strain VFN3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3089322..3099912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LUM42_RS14440 (LUM42_14440) aroB 3090252..3091337 (-) 1086 WP_004728570.1 3-dehydroquinate synthase -
  LUM42_RS14445 (LUM42_14445) aroK 3091365..3091883 (-) 519 WP_004728569.1 shikimate kinase AroK -
  LUM42_RS14450 (LUM42_14450) pilQ 3092071..3093795 (-) 1725 WP_004728568.1 type IV pilus secretin PilQ Machinery gene
  LUM42_RS14455 (LUM42_14455) pilP 3093820..3094332 (-) 513 WP_232658223.1 pilus assembly protein PilP Machinery gene
  LUM42_RS14460 (LUM42_14460) pilO 3094322..3094912 (-) 591 WP_232658224.1 type 4a pilus biogenesis protein PilO Machinery gene
  LUM42_RS14465 (LUM42_14465) pilN 3094905..3095495 (-) 591 WP_232658225.1 PilN domain-containing protein Machinery gene
  LUM42_RS14470 (LUM42_14470) pilM 3095485..3096489 (-) 1005 WP_232658226.1 type IV pilus assembly protein PilM Machinery gene
  LUM42_RS14475 (LUM42_14475) - 3096634..3099114 (+) 2481 WP_232658227.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22735.99 Da        Isoelectric Point: 5.0705

>NTDB_id=638366 LUM42_RS14460 WP_232658224.1 3094322..3094912(-) (pilO) [Vibrio furnissii strain VFN3]
MANVHDLELDEISEWPLVAQLVALVLLVLVLQGAGYWFYLQPKQDTLAARKQQEQTLKSTIRIKASKVATLPKLKIQLDE
LAQRYDFLLRQLPVQKELASMLASVNELGLDNSLTFTRIDWGEKQNQQFLYRLPLNIELTGNYHDIGDFSEAIARLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKPEVSDEQ

Nucleotide


Download         Length: 591 bp        

>NTDB_id=638366 LUM42_RS14460 WP_232658224.1 3094322..3094912(-) (pilO) [Vibrio furnissii strain VFN3]
ATGGCTAACGTACACGATCTGGAACTGGACGAAATCAGCGAATGGCCGTTGGTGGCTCAATTGGTCGCCTTGGTGCTGCT
GGTGTTGGTGTTGCAGGGCGCGGGATACTGGTTCTATTTGCAGCCTAAACAAGACACATTGGCCGCACGCAAGCAGCAAG
AGCAAACGCTGAAGTCGACGATTCGCATCAAAGCCAGCAAAGTCGCGACATTGCCGAAGCTCAAGATTCAGCTGGATGAA
CTGGCACAGCGGTACGACTTCCTGTTGCGTCAGCTGCCGGTGCAAAAAGAGCTCGCCAGCATGTTGGCGTCGGTGAACGA
GTTGGGGCTCGACAACAGCCTGACTTTTACTCGCATCGATTGGGGGGAAAAGCAGAATCAGCAATTTCTCTATCGGCTGC
CGCTCAATATCGAATTGACCGGTAATTATCACGACATCGGCGATTTTTCTGAAGCGATCGCGCGTCTACCGCGCATCATT
AATTTTGACGACGTTGACTGGCAGCGCGTCAGTCAAGAGAGCAGCACGCTCCATTTCCGCGTTCGCGCCTATACCTATCA
GTTCAAACCGGAGGTCAGTGATGAACAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

78.061

100

0.781
  pilO Vibrio campbellii strain DS40M4

70.526

96.939

0.684