Detailed information NTG0638015

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OCL45_RS10290
• Genome accession: NZ_CP106813
• Coordinates: 1950724..1951950 (-)
• Length: 408 a.a.
• NCBI ID: WP_263055886.1
• Uniprot ID: -
• Organism: Neisseria gonorrhoeae strain 9431
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1945724..1956950

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OCL45_RS10280 (OCL45_10280)	-	1947498..1947782 (+)	285	WP_003692093.1	GIY-YIG nuclease family protein	-
OCL45_RS10285 (OCL45_10285)	yccS	1948457..1950607 (-)	2151	WP_003697785.1	YccS family putative transporter	-
OCL45_RS10290 (OCL45_10290)	pilU	1950724..1951950 (-)	1227	WP_263055886.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OCL45_RS10295 (OCL45_10295)	pilT	1952114..1953157 (-)	1044	WP_003690200.1	type IV pilus twitching motility protein PilT	Machinery gene
OCL45_RS10300 (OCL45_10300)	-	1953277..1953963 (+)	687	WP_003690201.1	YggS family pyridoxal phosphate-dependent enzyme	-
OCL45_RS10305 (OCL45_10305)	-	1953963..1954370 (+)	408	WP_235220100.1	RNA-binding protein	-
OCL45_RS10310 (OCL45_10310)	proC	1954419..1955210 (+)	792	WP_260245430.1	pyrroline-5-carboxylate reductase	-
OCL45_RS10315 (OCL45_10315)	dksA	1955359..1955775 (+)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-
OCL45_RS10320 (OCL45_10320)	-	1955790..1955924 (+)	135	WP_263055887.1	hypothetical protein	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45601.16 Da
• Isoelectric Point: 6.9228

>NTDB_id=638015 OCL45_RS10290 WP_263055886.1 1950724..1951950(-) (pilU) [Neisseria gonorrhoeae strain 9431]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGTHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VSSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=638015 OCL45_RS10290 WP_263055886.1 1950724..1951950(-) (pilU) [Neisseria gonorrhoeae strain 9431]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCACACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCAGGAATTGGGCTTACCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCAGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGATCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.598	88.48	0.395
pilU	Pseudomonas stutzeri DSM 10701	44.944	87.255	0.392
pilU	Vibrio cholerae strain A1552	46.45	82.843	0.385