Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrA   Type   Machinery gene
Locus tag   LUZ06_RS10415 Genome accession   NZ_CP089493
Coordinates   2087784..2089133 (+) Length   449 a.a.
NCBI ID   WP_061731575.1    Uniprot ID   B4F4B7
Organism   Staphylococcus aureus strain 0308_IA_ST247     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 2069267..2110999 2087784..2089133 within 0


Gene organization within MGE regions


Location: 2069267..2110999
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LUZ06_RS10315 - 2069267..2071192 (-) 1926 WP_002485723.1 heavy metal translocating P-type ATPase -
  LUZ06_RS10320 - 2071522..2071848 (-) 327 WP_000391495.1 metalloregulator ArsR/SmtB family transcription factor -
  LUZ06_RS10325 - 2072055..2072414 (+) 360 WP_001631039.1 hypothetical protein -
  LUZ06_RS10330 - 2072526..2072864 (-) 339 Protein_1995 recombinase family protein -
  LUZ06_RS10335 - 2072873..2073502 (+) 630 Protein_1996 hypothetical protein -
  LUZ06_RS10340 ccrA 2073689..2075050 (+) 1362 WP_001631036.1 cassette chromosome recombinase CcrA Machinery gene
  LUZ06_RS10345 ccrB 2075047..2076675 (+) 1629 WP_001631035.1 cassette chromosome recombinase CcrB Machinery gene
  LUZ06_RS10350 - 2077176..2077526 (+) 351 WP_001631034.1 SAUGI family uracil-DNA glycosylase inhibitor -
  LUZ06_RS10355 - 2077613..2077924 (+) 312 WP_001631033.1 DUF960 domain-containing protein -
  LUZ06_RS10360 - 2077940..2078443 (+) 504 WP_001631032.1 DUF1643 domain-containing protein -
  LUZ06_RS10365 - 2078458..2078678 (+) 221 Protein_2002 hypothetical protein -
  LUZ06_RS10370 - 2078841..2079251 (+) 411 WP_001631031.1 DUF2871 domain-containing protein -
  LUZ06_RS10375 - 2079923..2080420 (+) 498 WP_001631030.1 GNAT family N-acetyltransferase -
  LUZ06_RS10380 - 2080479..2080739 (+) 261 Protein_2005 IS5/IS1182 family transposase -
  LUZ06_RS10385 - 2080743..2081945 (-) 1203 WP_061731577.1 restriction endonuclease subunit S -
  LUZ06_RS10390 - 2081938..2082081 (-) 144 WP_001631028.1 restriction endonuclease subunit R -
  LUZ06_RS10395 - 2082410..2083339 (-) 930 WP_231923532.1 AAA family ATPase -
  LUZ06_RS10400 - 2083323..2084093 (-) 771 WP_000767841.1 DUF6414 family protein -
  LUZ06_RS10405 - 2084210..2085640 (+) 1431 WP_001008608.1 DUF6038 family protein -
  LUZ06_RS10410 cch1 2085760..2087550 (+) 1791 WP_061731576.1 cassette chromosome replicative helicase -
  LUZ06_RS10415 ccrA 2087784..2089133 (+) 1350 WP_061731575.1 cassette chromosome recombinase CcrA Machinery gene
  LUZ06_RS10420 ccrB 2089155..2090783 (+) 1629 WP_001186602.1 cassette chromosome recombinase CcrB Machinery gene
  LUZ06_RS10425 - 2091301..2091651 (+) 351 WP_000859155.1 SAUGI family uracil-DNA glycosylase inhibitor -
  LUZ06_RS10430 - 2091738..2092049 (+) 312 WP_000700853.1 DUF960 family protein -
  LUZ06_RS10435 - 2092067..2092573 (+) 507 WP_001092169.1 DUF1643 domain-containing protein -
  LUZ06_RS10440 - 2092594..2092911 (+) 318 WP_000659050.1 JAB domain-containing protein -
  LUZ06_RS10445 - 2093030..2094115 (+) 1086 WP_000868132.1 tyrosine-type recombinase/integrase -
  LUZ06_RS10450 - 2094112..2096004 (+) 1893 WP_001557544.1 site-specific integrase -
  LUZ06_RS10455 - 2096011..2096388 (+) 378 WP_000361059.1 DUF6262 family protein -
  LUZ06_RS10460 - 2096539..2097321 (+) 783 WP_000067268.1 aminoglycoside nucleotidyltransferase ANT(9)-Ia -
  LUZ06_RS10465 erm(A) 2097447..2098178 (-) 732 WP_001072201.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(A) -
  LUZ06_RS10470 - 2098261..2098320 (-) 60 WP_010959190.1 erythromycin resistance leader peptide -
  LUZ06_RS10475 - 2098712..2099374 (+) 663 WP_001092058.1 class I SAM-dependent methyltransferase -
  LUZ06_RS10480 - 2099719..2100357 (-) 639 Protein_2025 sulfite exporter TauE/SafE family protein -
  LUZ06_RS10485 cstR 2100357..2100617 (-) 261 WP_000220507.1 persulfide-sensing transcriptional repressor CstR -
  LUZ06_RS10490 - 2100753..2101817 (+) 1065 WP_000438836.1 DsrE/DsrF/DrsH-like family protein -
  LUZ06_RS10495 cstB 2101850..2103184 (+) 1335 Protein_2028 persulfide dioxygenase-sulfurtransferase CstB -
  LUZ06_RS10500 - 2103298..2104428 (-) 1131 WP_010922808.1 ROK family transcriptional regulator -
  LUZ06_RS10505 psm-mec 2104762..2104830 (+) 69 WP_014532405.1 phenol-soluble modulin PSM-mec -
  LUZ06_RS10510 - 2104915..2105022 (-) 108 Protein_2031 BlaI/MecI/CopY family transcriptional regulator -
  LUZ06_RS10515 - 2105176..2106780 (+) 1605 WP_000284571.1 IS1182-like element IS1182 family transposase -
  LUZ06_RS10520 - 2106885..2107136 (-) 252 Protein_2033 BlaI/MecI/CopY family transcriptional regulator -
  LUZ06_RS10525 mecR1 2107136..2108893 (-) 1758 WP_000952923.1 beta-lactam sensor/signal transducer MecR1 -
  LUZ06_RS10530 mecA 2108990..2110999 (+) 2010 WP_001801873.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52557.24 Da        Isoelectric Point: 9.9150

>NTDB_id=637490 LUZ06_RS10415 WP_061731575.1 2087784..2089133(+) (ccrA) [Staphylococcus aureus strain 0308_IA_ST247]
MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQSGRKDNRSGYRQMTQLIQQGQCDILCCYRLNRLHRN
LKNALKLIKLCQTYHVHILSVHDGYFDMDQAFDRLKLNIFISLAELESDNIGEQVKNGLQEKAKQGQLITTHAPFGYDYH
NGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIMNPNYCGRVINQYGQFDNMFPSIVSANV
YEQAQRLRLQKQTKQTSSDNQLKQKIKCPCCNATLTNMTVRKKNHILRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEV
CRDFYQNQRIYTKIKGAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSINGHQIQHTIQ
NIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=637490 LUZ06_RS10415 WP_061731575.1 2087784..2089133(+) (ccrA) [Staphylococcus aureus strain 0308_IA_ST247]
ATGAAACAAGTTATAGGCTATTTACGTCAAAGTACGATGAAACAACAATCTCTTGCAGCACAGAAACAGGCTATCGAAGC
AATAGCCGAAAAACATCATATTCAACATATCAACTTTTATAGCGACAAACAATCAGGACGCAAAGATAATCGTAGTGGGT
ATCGACAAATGACGCAATTAATTCAACAAGGGCAATGTGACATATTATGTTGTTATCGTCTTAATAGATTACATCGTAAT
CTGAAAAATGCATTAAAACTCATCAAATTATGTCAAACTTATCATGTTCATATCTTAAGCGTACATGATGGTTACTTTGA
TATGGATCAAGCTTTCGACCGACTCAAGCTTAATATCTTCATCAGTTTAGCCGAACTTGAATCAGATAATATTGGAGAAC
AAGTCAAAAATGGCCTTCAAGAAAAGGCAAAACAAGGTCAATTGATTACAACACATGCGCCTTTTGGTTACGACTATCAC
AACGGAACATTCATCATCAATCAAAATGAGTCACCAACGGTAAAGGCTGTATTCAATTATTACATTAAAGGCCATGGTTA
TAAGAAAATTGCACAGTTATTAGAAGAAGATAACACGTATATCAATCGACAACCTTATCAAGTTCGTAATATTATCATGA
ACCCTAATTATTGTGGTCGTGTTATCAACCAATACGGTCAATTCGACAATATGTTCCCTTCTATTGTTTCAGCAAATGTA
TATGAACAAGCGCAGAGACTTCGATTGCAAAAACAAACCAAACAGACATCTTCGGATAATCAACTCAAACAAAAAATCAA
ATGCCCATGTTGTAATGCAACACTTACAAATATGACCGTTAGAAAAAAGAATCATATATTACGTTACTACGTCTGTCCTA
AAAATATGAATGCTTCACGCTTTGTCTGTGATTTCAAAGGCATCAATGCACAGACATTGGAAGATAAAGTATTAGAAGTG
TGCCGAGACTTTTATCAAAATCAACGCATCTACACAAAAATTAAAGGTGCGATTGACAAACGCATCAAAAGACAAAGAAA
CATAGAAAAACATCACACATTGACTCAAGAACAACTGATAGAAAAGTTGGCACAAGGCATCATCGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAACAACCGCAACGCACTACATCTATCAATGGACATCAAATACAACACACCATTCAA
AATATTATTCAAAAACGTTTCACGTTAAACATATTGTACCCCTATATTGAAACCATTCACATTACGAAAGATAAAAATCT
TATAGGAATCTATTTCAAAAATGAACCACTCAATATCGTCAATCAAACCATGCAATCATCGATTGCATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase (Recombinase)

(169-248)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B4F4B7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

96.659

100

0.967
  ccrA Staphylococcus aureus COL

75.056

100

0.751