Detailed information    

experimental Experimentally validated

Overview

Name   ccrA   Type   Machinery gene
Locus tag   SACOL_RS00225 Genome accession   NC_002951
Coordinates   47998..49347 (-) Length   449 a.a.
NCBI ID   WP_000806247.1    Uniprot ID   -
Organism   Staphylococcus aureus COL     
Function   promote SCCmec transfer   
Homologous recombination

Function

SCCmec carries the ccr genes which encode recombinases that allow its excision and integration at the attB attachment site in the recipient's chromosome.


Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 39643..49347 47998..49347 within 0


Gene organization within MGE regions


Location: 39643..49347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SACOL_RS00185 (SA0033) mecA 39643..41652 (-) 2010 WP_063852655.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  SACOL_RS00190 (SA0034) - 41749..42735 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  SACOL_RS00195 (SA0035) - 42707..42967 (+) 261 WP_001549961.1 type I restriction enzyme endonuclease domain-containing protein -
  SACOL_RS00200 (SA0036) - 42958..44565 (-) 1608 Protein_33 IS1182 family transposase -
  SACOL_RS00205 (SA0037) - 44617..45123 (-) 507 WP_001092195.1 DUF1643 domain-containing protein -
  SACOL_RS00210 (SA0038) - 45138..45449 (-) 312 WP_001024803.1 DUF960 family protein -
  SACOL_RS00215 (SA0040) - 45536..45886 (-) 351 WP_000859157.1 SAUGI family uracil-DNA glycosylase inhibitor -
  SACOL_RS00220 (SACOL0041) ccrB 46352..47976 (-) 1625 Protein_37 recombinase family protein Machinery gene
  SACOL_RS00225 (SA0042) ccrA 47998..49347 (-) 1350 WP_000806247.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 52580.41 Da        Isoelectric Point: 9.9370

>NTDB_id=1 SACOL_RS00225 WP_000806247.1 47998..49347(-) (ccrA) [Staphylococcus aureus COL]
MKQAIGYLRQSTTKQQSLAAQKQTIEALAKKHNIQYITFYSDKQSGRTDKRNGYQQITELIQQGQCDVLCCYRLNRLHRN
LKNALKLMKLCQKYHVHILSVHDGYFDMDKAFDRLKLNIFISLAELESDNIGEQVKNGIKEKAKQGKMITTHAPFGYHYH
NGTFTIDTVKAPTVKAVFNYYLQGYGYKKIAQYLEADDKFINRKPYQVRNIILNPNYCGRVINQYGQYENMFPAIVSTTI
YEEAQVTRTQKPVKRKPSENQLKQKIKCPYCDSTLTNMTIRKKHHTLRYYVCPQNMNASRFVCEFKGINAQELETSVLAT
CQDFFQNQQLYSKINHTIQQRLKRQRDIETKTTLNHEQLIEKLAQDKIDAETFREQTQSLRQQSKPISSISTYQIRKAFQ
NIIQQRFTLNMLYPYIDEINISKNKSLAGIYFKNEPLNIVKQTMELSIV

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=1 SACOL_RS00225 WP_000806247.1 47998..49347(-) (ccrA) [Staphylococcus aureus COL]
ATGAAACAAGCAATAGGTTACTTACGACAGAGCACTACAAAGCAACAATCCTTAGCAGCACAAAAACAAACCATCGAGGC
ATTAGCCAAAAAACATAATATTCAATACATTACCTTTTATAGCGATAAGCAATCAGGACGCACTGATAAGCGGAACGGTT
ACCAACAAATTACTGAACTGATTCAACAAGGACAATGTGATGTATTATGTTGTTACAGATTAAACCGACTTCATCGCAAT
CTTAAAAATGCATTAAAACTCATGAAATTGTGTCAAAAATACCATGTCCATATCTTAAGCGTTCATGATGGCTATTTTGA
TATGGATAAAGCATTCGATCGGCTCAAACTCAATATTTTCATCAGCTTGGCCGAACTAGAATCTGATAATATAGGCGAAC
AAGTCAAAAATGGAATCAAAGAAAAAGCGAAACAAGGTAAAATGATTACAACACATGCACCCTTTGGGTATCACTATCAT
AATGGTACTTTCACGATAGACACAGTAAAAGCACCAACAGTAAAAGCTGTGTTCAATTATTACCTTCAAGGTTATGGTTA
TAAAAAAATTGCGCAATACTTAGAAGCTGATGATAAATTCATTAATCGTAAGCCCTATCAAGTGCGTAATATTATCCTTA
ACCCTAATTACTGTGGCCGTGTTATCAATCAATACGGACAATATGAAAACATGTTCCCAGCTATTGTCAGTACAACGATA
TACGAAGAAGCTCAAGTTACCCGAACTCAGAAACCAGTAAAACGTAAGCCGTCAGAAAACCAACTCAAACAAAAAATCAA
ATGTCCTTATTGTGATTCAACACTAACTAATATGACCATTAGAAAAAAGCACCATACATTACGTTATTATGTTTGTCCTC
AAAATATGAATGCATCTCGTTTTGTCTGTGAATTCAAAGGGATCAACGCACAAGAATTAGAAACAAGTGTTTTAGCGACT
TGTCAGGACTTCTTTCAAAATCAACAGCTCTATTCAAAAATAAACCATACTATTCAACAACGACTCAAAAGACAAAGAGA
TATAGAAACTAAAACTACACTCAATCACGAGCAGCTGATAGAAAAATTAGCCCAAGACAAAATTGATGCAGAAACGTTCA
GAGAACAAACGCAATCATTACGTCAGCAATCAAAACCTATATCATCAATCAGTACGTATCAAATTCGAAAAGCTTTCCAA
AACATCATTCAACAACGTTTCACGCTAAACATGTTGTACCCCTATATTGATGAAATTAATATTTCTAAAAATAAAAGCCT
CGCTGGCATCTATTTCAAAAATGAACCACTGAATATTGTAAAGCAGACTATGGAATTATCAATTGTATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(4-147)

Recombinase (Recombinase)

(170-249)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(262-322)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrA Staphylococcus aureus N315

74.833

100

0.748

Multiple sequence alignment    



References

[1] Mais Maree et al. (2024) SCCmec transformation requires living donor cells in mixed biofilms. Biofilm 7:100184. [PMID: 38440091]