Detailed information    

experimental Experimentally validated

Overview

Name   ccrB   Type   Machinery gene
Locus tag   SACOL_RS00220 Genome accession   NC_002951
Coordinates   46352..47976 (-) Length   540 a.a.
NCBI ID   Protein_37    Uniprot ID   -
Organism   Staphylococcus aureus COL     
Function   promote SCCmec transfer   
Homologous recombination

Function

SCCmec carries the ccr genes which encode recombinases that allow its excision and integration at the attB attachment site in the recipient's chromosome.


Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 39643..49347 46352..47976 within 0


Gene organization within MGE regions


Location: 39643..49347
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SACOL_RS00185 (SA0033) mecA 39643..41652 (-) 2010 WP_063852655.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  SACOL_RS00190 (SA0034) - 41749..42735 (+) 987 WP_001549960.1 beta-lactam sensor/signal transducer MecR1 -
  SACOL_RS00195 (SA0035) - 42707..42967 (+) 261 WP_001549961.1 type I restriction enzyme endonuclease domain-containing protein -
  SACOL_RS00200 (SA0036) - 42958..44565 (-) 1608 Protein_33 IS1182 family transposase -
  SACOL_RS00205 (SA0037) - 44617..45123 (-) 507 WP_001092195.1 DUF1643 domain-containing protein -
  SACOL_RS00210 (SA0038) - 45138..45449 (-) 312 WP_001024803.1 DUF960 family protein -
  SACOL_RS00215 (SA0040) - 45536..45886 (-) 351 WP_000859157.1 SAUGI family uracil-DNA glycosylase inhibitor -
  SACOL_RS00220 (SACOL0041) ccrB 46352..47976 (-) 1625 Protein_37 recombinase family protein Machinery gene
  SACOL_RS00225 (SA0042) ccrA 47998..49347 (-) 1350 WP_000806247.1 cassette chromosome recombinase CcrA Machinery gene

Sequence

Protein


Download         Length: 540 a.a.        Molecular weight: Da        Isoelectric Point:

>NTDB_id=2 SACOL_RS00220 Protein_37 46352..47976(-) (ccrB) [Staphylococcus aureus COL]
None

Nucleotide


Download         Length: 1625 bp        

>NTDB_id=2 SACOL_RS00220 Protein_37 46352..47976(-) (ccrB) [Staphylococcus aureus COL]
ATGGATAAAATGAAGAAAAAGCTTGTAGGAGGCTACATTCGTGTGTCCACAGAGAGACAAGTAGAAGGTTATAGCATAGA
GGGACAAATTACACAAATAGAGCAATATTGCCAATTTAACGGCTATGAACTTGTTGATATATATGCAGATCGGGGTATAT
CAGGAAAATCTATGAACCGTCCTGAATTACAGCGCATGTTAAATGATGCTAAAAATGGAAAATTAGACTGTGTTATGGTT
TATAAAACAAATCGTTTGGCACGTAATACTTCCGATTTACTTACAATAGTCGAAGAACTTCATCGCCAAAATGTTGAATT
TTTTAGCTTGTCTGAACGTATGGAAGTCAAAAATTCAACAGGCAAGTTAATGCTCCAGATACTTGCAAGTTTTTCCGAAT
TCGAAAGAAATACAATTTTAGAGAATATTTACACCGGACAACGTCAAAGAGCTTTAGAGGGCTATTATCAAGGCAATCTT
CCATTAGGATATAATAACATACCTGATAATAAAAAAGAATTAATGATTAATCAACATGAAGCTAACATCGTTAAATATAT
CTTTGAATCTTATGCCAAAGGTCATGGTTATCGTAAAATAGCCAATGCATTCAATCACAAAGGCTATGTGACTAAAAAAG
GCAATCCATTTAGTATTTCAGCTGTTACTTATATTCTCTCAAACCCATTCTATATTGGTAAAATTCAATTCGCGAAATAC
AAAGATTGGAATGATAAACGACGTAAAGGATTAAACGATAAGCCAGTAATCGCTGAAGGTAAACACACGCCTATTATTAG
TCAATCATTATGGGATAAAGTGCAAGCACGTAAGAAACAAGTAAGTGAAAAACCACAAGTCCATGGTAAAGGAACCAATA
TTTTAACTGGAATAATTTCGTGTCCCCAATGTTCTGCACCTATGGCTGCGAGTAACACCACAAACACACTTAAAGATGGC
ACTAAAAACGTATTCGTTACTATTCGTGTAGTAATTTTCGAAATAAAGGCTCAAAGGTATGTTCTGCGAATAGTGTTAGA
GCCGATGTCATTGAAAAATATGTTATGGACCAAATACTTGAAATTGTCAAAAGTGATAAAGTTCTCAAACAAGTTGTCGA
ACGTGTCAATCAAGAGAATCAAGTCGATGTAGCTGCACTTAACCATGATATTGCTTATAAACAACAACAATTTGATGAAA
TTAGCACTAAACTTAAAAATCTAATTCAAACCATCGAAGACAATCCAGACTTAACATCTGCACTCAAACCAACCATTCAT
CAATATGAAACACAACTGAATGATATCACAAACCAAATTAATCAACTCAAGCACCAACAAAATCAAGAGAAACCATTTTA
TGATACTAAACAAATCGCTGCTTTATTACAACGAATATTTCAAAATATAGAATCCATGGATAAATCACAGCTCAAAGCTT
TGTACCTAACGGTTATTGACCGTATTGACATTCGTAAAGATGAGAATCATAAAAAGCAATTCTTTGTTACACTAAAACTT
AATAATGAAATTATTAAACAACTTTTCAATAATAATAATCTAGACGAAGTGCTTCTCAGCACTTCGTCTTTGTTTTTGCC
TCAAACACTCTACTTTCAAATATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Mais Maree et al. (2024) SCCmec transformation requires living donor cells in mixed biofilms. Biofilm 7:100184. [PMID: 38440091]