Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LLE38_RS10105 Genome accession   NZ_CP106777
Coordinates   1924766..1925992 (+) Length   408 a.a.
NCBI ID   WP_003692100.1    Uniprot ID   Q5F5L4
Organism   Neisseria gonorrhoeae strain 10727     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1919766..1930992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLE38_RS10075 (LLE38_10075) - 1920792..1920926 (-) 135 WP_258076284.1 hypothetical protein -
  LLE38_RS10080 (LLE38_10080) dksA 1920941..1921357 (-) 417 WP_003692108.1 RNA polymerase-binding protein DksA -
  LLE38_RS10085 (LLE38_10085) proC 1921506..1922297 (-) 792 WP_003694504.1 pyrroline-5-carboxylate reductase -
  LLE38_RS10090 (LLE38_10090) - 1922346..1922753 (-) 408 WP_003690202.1 hypothetical protein -
  LLE38_RS10095 (LLE38_10095) - 1922753..1923439 (-) 687 WP_003692103.1 YggS family pyridoxal phosphate-dependent enzyme -
  LLE38_RS10100 (LLE38_10100) pilT 1923559..1924602 (+) 1044 WP_003690200.1 type IV pilus twitching motility protein PilT Machinery gene
  LLE38_RS10105 (LLE38_10105) pilU 1924766..1925992 (+) 1227 WP_003692100.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LLE38_RS10110 (LLE38_10110) yccS 1926109..1928236 (+) 2128 Protein_1949 YccS family putative transporter -
  LLE38_RS10115 (LLE38_10115) - 1928718..1929002 (-) 285 WP_003692093.1 GIY-YIG nuclease family protein -
  LLE38_RS10120 (LLE38_10120) pilC 1929910..1930446 (-) 537 WP_263071067.1 PilC family type IV pilus tip adhesin Machinery gene

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45698.33 Da        Isoelectric Point: 7.2909

>NTDB_id=636957 LLE38_RS10105 WP_003692100.1 1924766..1925992(+) (pilU) [Neisseria gonorrhoeae strain 10727]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGTHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=636957 LLE38_RS10105 WP_003692100.1 1924766..1925992(+) (pilU) [Neisseria gonorrhoeae strain 10727]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCACACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCGGGAATTGGGCTTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAGGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(49-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5F5L4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

44.875

88.48

0.397
  pilU Pseudomonas stutzeri DSM 10701

45.225

87.255

0.395
  pilU Vibrio cholerae strain A1552

47.024

82.353

0.387