Detailed information NTG0636812

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LLE35_RS10215
• Genome accession: NZ_CP106773
• Coordinates: 1930768..1931994 (+)
• Length: 408 a.a.
• NCBI ID: WP_003692100.1
• Uniprot ID: Q5F5L4
• Organism: Neisseria gonorrhoeae strain 10562
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1925768..1936994

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLE35_RS10190 (LLE35_10115)	dksA	1926943..1927359 (-)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-
LLE35_RS10195 (LLE35_10120)	proC	1927508..1928299 (-)	792	WP_003694504.1	pyrroline-5-carboxylate reductase	-
LLE35_RS10200 (LLE35_10125)	-	1928348..1928755 (-)	408	WP_003690202.1	hypothetical protein	-
LLE35_RS10205 (LLE35_10130)	-	1928755..1929441 (-)	687	WP_003692103.1	YggS family pyridoxal phosphate-dependent enzyme	-
LLE35_RS10210 (LLE35_10135)	pilT	1929561..1930604 (+)	1044	WP_003690200.1	type IV pilus twitching motility protein PilT	Machinery gene
LLE35_RS10215 (LLE35_10140)	pilU	1930768..1931994 (+)	1227	WP_003692100.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LLE35_RS10220 (LLE35_10145)	yccS	1932111..1934261 (+)	2151	WP_003692098.1	YccS family putative transporter	-
LLE35_RS10225 (LLE35_10150)	-	1934743..1935027 (-)	285	WP_003692093.1	GIY-YIG nuclease family protein	-
LLE35_RS11900	pilC	1935934..1936218 (-)	285	WP_317629693.1	hypothetical protein	Machinery gene
LLE35_RS11905	pilC	1936224..1936469 (-)	246	WP_317629694.1	hypothetical protein	Machinery gene

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45698.33 Da
• Isoelectric Point: 7.2909

>NTDB_id=636812 LLE35_RS10215 WP_003692100.1 1930768..1931994(+) (pilU) [Neisseria gonorrhoeae strain 10562]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGTHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=636812 LLE35_RS10215 WP_003692100.1 1930768..1931994(+) (pilU) [Neisseria gonorrhoeae strain 10562]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCACACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCGGGAATTGGGCTTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAGGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: Q5F5L4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.875	88.48	0.397
pilU	Pseudomonas stutzeri DSM 10701	45.225	87.255	0.395
pilU	Vibrio cholerae strain A1552	47.024	82.353	0.387