Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LT232_RS00875 Genome accession   NZ_CP089287
Coordinates   145595..146665 (+) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain GSBZ09     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 140595..151665
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LT232_RS00845 (LT232_00845) - 141066..141311 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  LT232_RS00850 (LT232_00850) - 141549..141929 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  LT232_RS00860 (LT232_00860) - 142136..142966 (+) 831 WP_007408317.1 STAS domain-containing protein -
  LT232_RS00865 (LT232_00865) - 143004..144305 (-) 1302 WP_021494315.1 hemolysin family protein -
  LT232_RS00870 (LT232_00870) - 144451..145401 (+) 951 WP_007408319.1 magnesium transporter CorA family protein -
  LT232_RS00875 (LT232_00875) comGA 145595..146665 (+) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  LT232_RS00880 (LT232_00880) comGB 146652..147689 (+) 1038 WP_094031834.1 competence type IV pilus assembly protein ComGB Machinery gene
  LT232_RS00885 (LT232_00885) comGC 147694..148002 (+) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  LT232_RS00890 (LT232_00890) comGD 147992..148429 (+) 438 WP_043020787.1 competence type IV pilus minor pilin ComGD Machinery gene
  LT232_RS00895 (LT232_00895) comGE 148413..148727 (+) 315 WP_012117982.1 competence type IV pilus minor pilin ComGE -
  LT232_RS00900 (LT232_00900) comGF 148636..149136 (+) 501 WP_257899725.1 competence type IV pilus minor pilin ComGF -
  LT232_RS00905 (LT232_00905) comGG 149137..149514 (+) 378 WP_012117980.1 competence type IV pilus minor pilin ComGG Machinery gene
  LT232_RS00910 (LT232_00910) - 149571..149750 (+) 180 WP_003153093.1 YqzE family protein -
  LT232_RS00915 (LT232_00915) - 149790..150119 (-) 330 WP_012117979.1 DUF3889 domain-containing protein -
  LT232_RS00920 (LT232_00920) tapA 150378..151049 (+) 672 WP_124934997.1 amyloid fiber anchoring/assembly protein TapA -
  LT232_RS00925 (LT232_00925) sipW 151021..151605 (+) 585 WP_015240205.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=636129 LT232_RS00875 WP_003153083.1 145595..146665(+) (comGA) [Bacillus velezensis strain GSBZ09]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=636129 LT232_RS00875 WP_003153083.1 145595..146665(+) (comGA) [Bacillus velezensis strain GSBZ09]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTAAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374