Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PALA14_RS05445 Genome accession   NZ_CP106742
Coordinates   1156126..1156749 (+) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain PALA14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1151126..1161749
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA14_RS05430 (PALA14_01070) - 1151813..1154284 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  PALA14_RS05435 (PALA14_01071) pilM 1154469..1155533 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  PALA14_RS05440 (PALA14_01072) pilN 1155533..1156129 (+) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  PALA14_RS05445 (PALA14_01073) pilO 1156126..1156749 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  PALA14_RS05450 (PALA14_01074) comL/pilP 1156746..1157270 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  PALA14_RS05455 (PALA14_01075) pilQ 1157324..1159468 (+) 2145 WP_023465329.1 type 4a pilus secretin PilQ Machinery gene
  PALA14_RS05460 (PALA14_01076) aroK 1159480..1159998 (+) 519 WP_003095833.1 shikimate kinase AroK -
  PALA14_RS05465 (PALA14_01077) aroB 1160048..1161154 (+) 1107 WP_003162482.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=635916 PALA14_RS05445 WP_003103268.1 1156126..1156749(+) (pilO) [Pseudomonas aeruginosa strain PALA14]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=635916 PALA14_RS05445 WP_003103268.1 1156126..1156749(+) (pilO) [Pseudomonas aeruginosa strain PALA14]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401