Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   LTQ03_RS08280 Genome accession   NZ_CP089201
Coordinates   1839080..1839709 (+) Length   209 a.a.
NCBI ID   WP_004729667.1    Uniprot ID   -
Organism   Vibrio splendidus strain 1_C04a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1834080..1844709
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LTQ03_RS08265 (LTQ03_08265) - 1834766..1837327 (-) 2562 WP_243583168.1 PBP1A family penicillin-binding protein -
  LTQ03_RS08270 (LTQ03_08270) pilM 1837479..1838504 (+) 1026 WP_004729669.1 type IV pilus assembly protein PilM Machinery gene
  LTQ03_RS08275 (LTQ03_08275) pilN 1838488..1839087 (+) 600 WP_102481610.1 PilN domain-containing protein Machinery gene
  LTQ03_RS08280 (LTQ03_08280) pilO 1839080..1839709 (+) 630 WP_004729667.1 type 4a pilus biogenesis protein PilO Machinery gene
  LTQ03_RS08285 (LTQ03_08285) pilP 1839696..1840214 (+) 519 WP_004729666.1 pilus assembly protein PilP Machinery gene
  LTQ03_RS08290 (LTQ03_08290) pilQ 1840250..1841968 (+) 1719 WP_243583170.1 type IV pilus secretin PilQ family protein Machinery gene
  LTQ03_RS08295 (LTQ03_08295) aroK 1842145..1842663 (+) 519 WP_004729664.1 shikimate kinase AroK -
  LTQ03_RS08300 (LTQ03_08300) aroB 1842689..1843777 (+) 1089 WP_004729663.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 209 a.a.        Molecular weight: 24294.86 Da        Isoelectric Point: 4.6751

>NTDB_id=635656 LTQ03_RS08280 WP_004729667.1 1839080..1839709(+) (pilO) [Vibrio splendidus strain 1_C04a]
MANLQNKISWQNRMSLQDLDVDEITEWPLLPQLLVILVLIVLIQGVGTWLYVLPLDDELQQMKQQEQTLKATLRIKANKV
AALPKLQSQLDELTSRYDYLLEQLPVQKELASMLASVNELGLDNKLTFTRIDWGQKQNKEFLYRLPLNIELTGDYHEIGD
FSAAIAKLPRIISFDDVNWQRVSQESSTLHFRVRAYTYQFKSEVNDETQ

Nucleotide


Download         Length: 630 bp        

>NTDB_id=635656 LTQ03_RS08280 WP_004729667.1 1839080..1839709(+) (pilO) [Vibrio splendidus strain 1_C04a]
ATGGCTAACCTTCAAAATAAGATTAGTTGGCAAAACAGGATGAGTTTGCAAGATCTCGATGTTGATGAGATCACCGAATG
GCCATTGTTGCCTCAGCTTCTCGTGATTCTGGTGTTGATAGTGTTAATCCAAGGTGTTGGCACTTGGCTTTATGTGCTGC
CGCTCGACGATGAACTGCAACAGATGAAGCAACAGGAGCAGACCTTAAAAGCGACCTTGAGAATAAAGGCCAATAAGGTT
GCGGCCTTACCTAAGCTTCAGAGCCAGCTGGATGAGCTAACTAGCCGTTATGACTATCTATTGGAGCAATTGCCCGTTCA
AAAAGAGCTAGCCAGTATGTTGGCCTCTGTGAACGAGCTTGGATTGGATAATAAGCTGACATTTACGCGAATTGACTGGG
GGCAGAAGCAGAACAAAGAGTTTCTCTATCGTTTACCGCTCAACATCGAGCTGACGGGTGACTACCACGAGATTGGTGAT
TTCTCTGCGGCCATTGCTAAGCTGCCACGCATCATCAGCTTTGATGACGTGAATTGGCAGCGAGTCAGTCAAGAAAGTAG
CACGCTGCACTTCAGGGTTCGAGCTTATACCTACCAATTTAAATCGGAGGTCAATGATGAAACCCAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(59-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

76.166

92.344

0.703
  pilO Vibrio campbellii strain DS40M4

73.514

88.517

0.651