Detailed information NTG0635654

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: LTQ03_RS08270
• Genome accession: NZ_CP089201
• Coordinates: 1837479..1838504 (+)
• Length: 341 a.a.
• NCBI ID: WP_004729669.1
• Uniprot ID: A0A7W4GL85
• Organism: Vibrio splendidus strain 1_C04a
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1832479..1843504

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LTQ03_RS08250 (LTQ03_08250)	-	1832826..1833554 (-)	729	WP_004729672.1	glutathione peroxidase	-
LTQ03_RS08255 (LTQ03_08255)	-	1833598..1833777 (-)	180	WP_017080197.1	hypothetical protein	-
LTQ03_RS08260 (LTQ03_08260)	oxyR	1833776..1834669 (+)	894	WP_243583167.1	DNA-binding transcriptional regulator OxyR	-
LTQ03_RS08265 (LTQ03_08265)	-	1834766..1837327 (-)	2562	WP_243583168.1	PBP1A family penicillin-binding protein	-
LTQ03_RS08270 (LTQ03_08270)	pilM	1837479..1838504 (+)	1026	WP_004729669.1	type IV pilus assembly protein PilM	Machinery gene
LTQ03_RS08275 (LTQ03_08275)	pilN	1838488..1839087 (+)	600	WP_102481610.1	PilN domain-containing protein	Machinery gene
LTQ03_RS08280 (LTQ03_08280)	pilO	1839080..1839709 (+)	630	WP_004729667.1	type 4a pilus biogenesis protein PilO	Machinery gene
LTQ03_RS08285 (LTQ03_08285)	pilP	1839696..1840214 (+)	519	WP_004729666.1	pilus assembly protein PilP	Machinery gene
LTQ03_RS08290 (LTQ03_08290)	pilQ	1840250..1841968 (+)	1719	WP_243583170.1	type IV pilus secretin PilQ family protein	Machinery gene
LTQ03_RS08295 (LTQ03_08295)	aroK	1842145..1842663 (+)	519	WP_004729664.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 341 a.a.
• Molecular weight: 38112.68 Da
• Isoelectric Point: 7.0208

>NTDB_id=635654 LTQ03_RS08270 WP_004729669.1 1837479..1838504(+) (pilM) [Vibrio splendidus strain 1_C04a]
MGSSLITGIDINHHSIKAVVLKPVGELYALVGYKELPISDDIFTANHTLEYQKTVKKLKELRKGLPFGCCNVAISVPDNT
VISKVLQIESELEDREKEFSIYQTFAHQSPFPIEELSLDFVKLEDKRFGKGSTSSYQVYATRKEVVDSRADALTKAGFKP
VVVDTQAHGLLNIWQMASRLYPEKKNWLLVDVGIDQTSLGIIPQGLAPFYKDIAFGTKDLRSHGAASDIESVFATTEDTH
QFIVNLIEKLKRQLQLYSSGNAQQPISGIWLMGEGASIPMVTEELERHFQMSCESLNPLSLFENKVAKRRRLPTNWQHFG
IAAGMAMSGLKWQGEKHVASN

Nucleotide

• Length: 1026 bp

>NTDB_id=635654 LTQ03_RS08270 WP_004729669.1 1837479..1838504(+) (pilM) [Vibrio splendidus strain 1_C04a]
ATGGGTTCATCATTAATTACAGGTATAGATATAAATCATCACAGCATCAAAGCCGTGGTACTAAAACCCGTGGGGGAGCT
GTATGCCCTAGTGGGATACAAAGAGCTGCCGATTTCGGACGACATTTTTACAGCTAACCATACTTTGGAGTATCAGAAAA
CTGTTAAGAAACTTAAAGAACTTAGGAAAGGGCTGCCTTTTGGCTGTTGCAACGTCGCCATTTCAGTACCTGATAACACG
GTGATCAGCAAAGTACTGCAAATAGAGAGTGAGTTAGAAGATAGAGAAAAAGAGTTTTCGATCTATCAAACCTTTGCTCA
TCAATCTCCCTTTCCGATAGAGGAGCTGAGTTTAGATTTTGTAAAGCTAGAAGACAAACGATTTGGTAAAGGTTCGACAA
GCAGTTATCAGGTATACGCCACTCGTAAAGAAGTGGTGGATAGCCGAGCGGATGCTTTGACCAAAGCTGGTTTTAAACCT
GTCGTGGTAGACACGCAGGCGCATGGGCTGCTTAACATCTGGCAAATGGCATCGCGCCTGTACCCCGAAAAGAAAAATTG
GCTGCTGGTGGATGTGGGAATCGACCAAACGTCGTTAGGCATTATTCCACAAGGTTTGGCACCGTTTTATAAAGATATCG
CTTTTGGTACCAAAGATCTTCGTAGCCATGGTGCTGCAAGTGATATCGAGAGTGTGTTCGCCACCACTGAAGATACACAT
CAATTTATTGTTAACCTGATTGAGAAGCTCAAGCGTCAATTGCAGCTCTATTCCTCGGGAAATGCGCAGCAACCTATCTC
AGGGATCTGGCTGATGGGCGAGGGCGCCAGCATTCCTATGGTAACCGAAGAGCTAGAACGTCATTTTCAGATGAGCTGCG
AGTCATTGAATCCTTTGTCTCTATTTGAGAATAAGGTGGCGAAGCGACGTCGATTACCAACGAATTGGCAACATTTCGGT
ATTGCGGCGGGTATGGCGATGAGTGGACTCAAGTGGCAGGGAGAAAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (242-328)

• Type IV pilus assembly protein PilM; (PilM_2) (8-220)

3D structure

• AlphaFold DB: A0A7W4GL85

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	53.824	99.707	0.537
pilM	Vibrio campbellii strain DS40M4	47.368	100	0.475