Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   N8E87_RS00185 Genome accession   NZ_CP104917
Coordinates   40526..41068 (+) Length   180 a.a.
NCBI ID   WP_017805302.1    Uniprot ID   -
Organism   Avibacterium paragallinarum strain AP-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 35526..46068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8E87_RS00160 (N8E87_00160) - 35982..36911 (+) 930 WP_264245175.1 phosphatidate cytidylyltransferase -
  N8E87_RS00165 (N8E87_00165) mog 36930..37508 (+) 579 WP_264245177.1 molybdopterin adenylyltransferase -
  N8E87_RS00170 (N8E87_00170) glnB 37561..37899 (+) 339 WP_264245179.1 nitrogen regulatory protein P-II -
  N8E87_RS00175 (N8E87_00175) - 37902..38954 (+) 1053 WP_264245181.1 AI-2E family transporter -
  N8E87_RS00180 (N8E87_00180) - 39323..40492 (+) 1170 WP_264245183.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  N8E87_RS00185 (N8E87_00185) pilF/pilF2 40526..41068 (+) 543 WP_017805302.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  N8E87_RS00190 (N8E87_00190) dprA 41213..42313 (+) 1101 WP_264245186.1 DNA-processing protein DprA Machinery gene
  N8E87_RS00195 (N8E87_00195) rsmD 42310..42918 (-) 609 WP_264245187.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  N8E87_RS00200 (N8E87_00200) pilA 43074..44492 (+) 1419 WP_264245189.1 signal recognition particle-docking protein FtsY Machinery gene

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20715.73 Da        Isoelectric Point: 8.0752

>NTDB_id=635153 N8E87_RS00185 WP_017805302.1 40526..41068(+) (pilF/pilF2) [Avibacterium paragallinarum strain AP-2]
MKIYQCILSILFSLFLVACVSTPLNKQDFDKQQAAKARVELGIGYLEQNELGLAKQNLDKALAYAPNYYLVHSALAYFYQ
LQGENTRAENAYLKALKLDDKQGDVFNNYAVFLCKQGQFEQAYLRFEQALATPNYYQQADTYENIVLCALAEKNAIRYEK
YKGLLEKLDPKRNQKTNVIH

Nucleotide


Download         Length: 543 bp        

>NTDB_id=635153 N8E87_RS00185 WP_017805302.1 40526..41068(+) (pilF/pilF2) [Avibacterium paragallinarum strain AP-2]
ATGAAAATTTATCAATGTATCTTAAGCATTCTTTTTTCTCTCTTTCTCGTAGCCTGTGTTTCCACTCCCTTAAATAAGCA
AGACTTTGATAAGCAGCAAGCCGCAAAGGCGAGAGTGGAACTTGGCATTGGCTATCTTGAGCAAAATGAACTTGGTCTGG
CGAAGCAAAATTTAGACAAAGCCTTAGCTTATGCCCCGAATTATTATTTAGTGCATTCAGCGTTGGCATATTTTTATCAG
CTTCAAGGGGAGAATACTCGTGCGGAAAATGCGTATTTGAAAGCCCTAAAACTCGACGATAAACAAGGCGATGTATTTAA
TAATTATGCCGTCTTTTTGTGCAAACAAGGACAATTCGAACAGGCTTATCTACGATTTGAACAAGCTCTCGCCACACCTA
ATTATTATCAGCAGGCGGATACTTACGAGAATATTGTACTTTGTGCGTTAGCAGAGAAAAATGCAATACGTTATGAAAAA
TATAAAGGCTTGCTGGAGAAACTAGATCCTAAAAGAAATCAAAAGACAAATGTTATTCATTAA

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

50.292

95

0.478
  pilF Glaesserella parasuis strain SC1401

43.407

100

0.439