Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   N8E86_RS00185 Genome accession   NZ_CP104914
Coordinates   40577..41119 (+) Length   180 a.a.
NCBI ID   WP_017805302.1    Uniprot ID   -
Organism   Avibacterium paragallinarum strain AG21-0333     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 35577..46119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8E86_RS00160 (N8E86_00160) - 36054..36983 (+) 930 WP_115249942.1 phosphatidate cytidylyltransferase -
  N8E86_RS00165 (N8E86_00165) mog 37002..37580 (+) 579 WP_264256079.1 molybdopterin adenylyltransferase -
  N8E86_RS00170 (N8E86_00170) glnB 37609..37947 (+) 339 WP_017805299.1 nitrogen regulatory protein P-II -
  N8E86_RS00175 (N8E86_00175) - 37950..39002 (+) 1053 WP_264256080.1 AI-2E family transporter -
  N8E86_RS00180 (N8E86_00180) - 39374..40543 (+) 1170 WP_264256081.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  N8E86_RS00185 (N8E86_00185) pilF/pilF2 40577..41119 (+) 543 WP_017805302.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  N8E86_RS00190 (N8E86_00190) dprA 41264..42364 (+) 1101 WP_110479718.1 DNA-processing protein DprA Machinery gene
  N8E86_RS00195 (N8E86_00195) rsmD 42361..42969 (-) 609 WP_264256082.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  N8E86_RS00200 (N8E86_00200) pilA 43125..44468 (+) 1344 WP_264256083.1 signal recognition particle-docking protein FtsY Machinery gene
  N8E86_RS00205 (N8E86_00205) glpQ 45014..46108 (-) 1095 WP_264256084.1 glycerophosphodiester phosphodiesterase -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20715.73 Da        Isoelectric Point: 8.0752

>NTDB_id=635099 N8E86_RS00185 WP_017805302.1 40577..41119(+) (pilF/pilF2) [Avibacterium paragallinarum strain AG21-0333]
MKIYQCILSILFSLFLVACVSTPLNKQDFDKQQAAKARVELGIGYLEQNELGLAKQNLDKALAYAPNYYLVHSALAYFYQ
LQGENTRAENAYLKALKLDDKQGDVFNNYAVFLCKQGQFEQAYLRFEQALATPNYYQQADTYENIVLCALAEKNAIRYEK
YKGLLEKLDPKRNQKTNVIH

Nucleotide


Download         Length: 543 bp        

>NTDB_id=635099 N8E86_RS00185 WP_017805302.1 40577..41119(+) (pilF/pilF2) [Avibacterium paragallinarum strain AG21-0333]
ATGAAAATTTATCAATGTATCTTAAGCATTCTTTTTTCTCTCTTTCTCGTAGCCTGTGTTTCCACTCCCTTAAATAAGCA
AGACTTTGATAAGCAGCAAGCCGCAAAGGCGAGAGTGGAGCTTGGCATTGGCTATCTTGAGCAAAATGAACTTGGTCTGG
CGAAGCAAAATTTAGACAAAGCTTTAGCTTATGCCCCGAATTATTATTTAGTGCATTCAGCATTGGCATATTTTTATCAG
CTTCAAGGGGAGAATACTCGTGCGGAAAATGCGTATTTGAAAGCCCTAAAACTCGACGATAAACAAGGTGATGTATTTAA
TAATTATGCCGTCTTTTTGTGCAAACAAGGACAATTCGAACAGGCTTATCTACGATTTGAACAAGCTCTCGCCACACCTA
ATTATTATCAGCAAGCGGATACTTACGAGAATATTGTACTTTGTGCGTTAGCAGAGAAAAATGCAATACGTTATGAAAAA
TATAAAGGCTTGCTGGAGAAACTAGATCCTAAAAGAAATCAAAAGACAAATGTTATTCATTAA

Domains

Predicted by InterProScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

50.292

95

0.478
  pilF Glaesserella parasuis strain SC1401

43.407

100

0.439