Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiA   Type   Regulator
Locus tag   CSB63_RS06975 Genome accession   NZ_CP089060
Coordinates   1312742..1314709 (-) Length   655 a.a.
NCBI ID   WP_134973353.1    Uniprot ID   -
Organism   Streptococcus thermophilus strain CH8     
Function   internalize XIP (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1292716..1362568 1312742..1314709 within 0
IScluster/Tn 1315065..1322430 1312742..1314709 flank 356


Gene organization within MGE regions


Location: 1292716..1362568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSB63_RS06880 (CSB63_1442) liaF 1292724..1293421 (-) 698 Protein_1300 cell wall-active antibiotics response protein LiaF -
  CSB63_RS06885 - 1293687..1293842 (-) 156 WP_011226290.1 ion channel -
  CSB63_RS06890 (CSB63_1444) stkP/pknB 1294479..1296350 (-) 1872 WP_024009998.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  CSB63_RS06895 (CSB63_1445) - 1296350..1297087 (-) 738 WP_134973348.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  CSB63_RS06900 (CSB63_1446) rsmB 1297131..1298453 (-) 1323 WP_014608533.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  CSB63_RS06905 (CSB63_1447) fmt 1298443..1299378 (-) 936 WP_002951411.1 methionyl-tRNA formyltransferase -
  CSB63_RS06910 (CSB63_1448) - 1299396..1301792 (-) 2397 WP_134973349.1 primosomal protein N' -
  CSB63_RS06915 (CSB63_1449) rpoZ 1301934..1302248 (-) 315 WP_041829315.1 DNA-directed RNA polymerase subunit omega -
  CSB63_RS06920 (CSB63_1450) gmk 1302270..1302899 (-) 630 WP_002951416.1 guanylate kinase -
  CSB63_RS06925 (CSB63_1451) ftsY 1303125..1304516 (-) 1392 WP_134973350.1 signal recognition particle-docking protein FtsY -
  CSB63_RS06930 (CSB63_1452) - 1304530..1305345 (-) 816 WP_002951420.1 Cof-type HAD-IIB family hydrolase -
  CSB63_RS06935 (CSB63_1453) - 1305338..1306132 (-) 795 WP_024010002.1 HAD-IIB family hydrolase -
  CSB63_RS06940 (CSB63_1454) - 1306317..1306694 (+) 378 WP_024010003.1 GntR family transcriptional regulator -
  CSB63_RS06945 (CSB63_1455) - 1306699..1307397 (+) 699 WP_134973351.1 ABC transporter ATP-binding protein -
  CSB63_RS06950 (CSB63_1456) - 1307409..1308194 (+) 786 WP_002951425.1 hypothetical protein -
  CSB63_RS06955 (CSB63_1457) amiF 1308244..1309173 (-) 930 WP_011681415.1 ATP-binding cassette domain-containing protein Regulator
  CSB63_RS06960 (CSB63_1458) amiE 1309166..1310251 (-) 1086 WP_011226304.1 ABC transporter ATP-binding protein Regulator
  CSB63_RS06965 (CSB63_1459) amiD 1310261..1311187 (-) 927 WP_134973352.1 oligopeptide ABC transporter permease OppC Regulator
  CSB63_RS06970 (CSB63_1460) amiC 1311187..1312680 (-) 1494 WP_065972442.1 ABC transporter permease Regulator
  CSB63_RS06975 (CSB63_1461) amiA 1312742..1314709 (-) 1968 WP_134973353.1 peptide ABC transporter substrate-binding protein Regulator
  CSB63_RS06980 - 1315065..1316316 (+) 1252 Protein_1320 ISL3 family transposase -
  CSB63_RS06985 (CSB63_1463) amiA3 1316361..1318334 (-) 1974 WP_134973354.1 peptide ABC transporter substrate-binding protein Regulator
  CSB63_RS06990 - 1318540..1318730 (-) 191 Protein_1322 IS3 family transposase -
  CSB63_RS10090 - 1318724..1319134 (-) 411 Protein_1323 ATP-binding cassette domain-containing protein -
  CSB63_RS10095 (CSB63_1466) - 1319166..1319479 (-) 314 Protein_1324 TatD family hydrolase -
  CSB63_RS07005 (CSB63_1467) - 1319825..1321030 (+) 1206 WP_011227415.1 L-lactate MFS transporter -
  CSB63_RS07010 - 1321077..1322430 (-) 1354 Protein_1326 IS3 family transposase -
  CSB63_RS07015 (CSB63_1473) pta 1322516..1323499 (-) 984 WP_134973355.1 phosphate acetyltransferase -
  CSB63_RS07020 (CSB63_1474) - 1323513..1324409 (-) 897 WP_011226316.1 RluA family pseudouridine synthase -
  CSB63_RS07025 (CSB63_1475) - 1324406..1325242 (-) 837 WP_065972449.1 NAD kinase -
  CSB63_RS07030 (CSB63_1476) - 1325214..1325888 (-) 675 WP_011681427.1 GTP pyrophosphokinase -
  CSB63_RS07035 (CSB63_1477) - 1325984..1326559 (+) 576 WP_002951444.1 CYTH domain-containing protein -
  CSB63_RS07040 (CSB63_1479) - 1326924..1327895 (+) 972 WP_002951446.1 ribose-phosphate diphosphokinase -
  CSB63_RS07045 (CSB63_1480) - 1327899..1329011 (+) 1113 WP_002948029.1 cysteine desulfurase family protein -
  CSB63_RS07050 (CSB63_1481) - 1329013..1329360 (+) 348 WP_002948028.1 DUF1831 domain-containing protein -
  CSB63_RS07055 (CSB63_1482) - 1329504..1330163 (+) 660 WP_071417293.1 redox-sensing transcriptional repressor Rex -
  CSB63_RS07060 (CSB63_1483) - 1330156..1330851 (+) 696 WP_002951449.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
  CSB63_RS07065 (CSB63_1484) radC 1330904..1331590 (-) 687 WP_011226324.1 RadC family protein -
  CSB63_RS07070 (CSB63_1485) - 1331639..1333423 (-) 1785 WP_134973356.1 rhamnan synthesis F family protein -
  CSB63_RS07075 (CSB63_1486) - 1333420..1334475 (-) 1056 WP_002948022.1 glycosyltransferase family 2 protein -
  CSB63_RS07080 (CSB63_1487) - 1334495..1335700 (-) 1206 WP_113870315.1 ABC transporter ATP-binding protein -
  CSB63_RS07085 (CSB63_1488) - 1335700..1336509 (-) 810 WP_002948020.1 ABC transporter permease -
  CSB63_RS07090 (CSB63_1489) - 1336493..1337449 (-) 957 WP_011226328.1 glycosyltransferase family 2 protein -
  CSB63_RS07095 (CSB63_1490) cps2T 1337446..1338594 (-) 1149 WP_041828290.1 beta 1-4 rhamnosyltransferase Cps2T -
  CSB63_RS07100 (CSB63_1491) - 1338701..1340242 (-) 1542 WP_134973357.1 DUF2142 domain-containing protein -
  CSB63_RS07105 (CSB63_1492) - 1340249..1341253 (-) 1005 WP_100284794.1 glycosyltransferase family 2 protein -
  CSB63_RS07110 (CSB63_1493) - 1341253..1342527 (-) 1275 WP_134973359.1 lipopolysaccharide biosynthesis protein -
  CSB63_RS07115 (CSB63_1494) - 1342520..1342852 (-) 333 WP_011226332.1 DUF2304 domain-containing protein -
  CSB63_RS07120 (CSB63_1495) - 1342858..1343553 (-) 696 WP_173405413.1 glycosyltransferase family 2 protein -
  CSB63_RS07125 (CSB63_1496) rfbD 1343628..1344479 (-) 852 WP_002951456.1 dTDP-4-dehydrorhamnose reductase -
  CSB63_RS07130 (CSB63_1497) - 1344564..1346038 (-) 1475 Protein_1350 glucosyltransferase domain-containing protein -
  CSB63_RS07135 (CSB63_1499) - 1346043..1346969 (-) 927 WP_134973361.1 glycosyltransferase family 2 protein -
  CSB63_RS07140 (CSB63_1500) - 1346980..1347315 (-) 336 WP_002891474.1 metal-sulfur cluster assembly factor -
  CSB63_RS07145 (CSB63_1501) rpoD 1347369..1348478 (-) 1110 WP_011227440.1 RNA polymerase sigma factor RpoD -
  CSB63_RS07150 (CSB63_1502) dnaG 1348482..1350293 (-) 1812 WP_011681448.1 DNA primase -
  CSB63_RS07155 (CSB63_1503) mscL 1350436..1350516 (+) 81 Protein_1355 large conductance mechanosensitive channel protein MscL -
  CSB63_RS07160 (CSB63_1504) rpsU 1350676..1350852 (-) 177 WP_011681449.1 30S ribosomal protein S21 -
  CSB63_RS07165 (CSB63_1505) - 1351047..1351841 (-) 795 WP_023909836.1 ABC transporter substrate-binding protein -
  CSB63_RS07170 (CSB63_1506) - 1351903..1353012 (-) 1110 WP_024703959.1 aminotransferase -
  CSB63_RS07175 (CSB63_1507) - 1353359..1354156 (-) 798 WP_134973363.1 transporter substrate-binding domain-containing protein -
  CSB63_RS07180 (CSB63_1508) - 1354153..1354983 (-) 831 WP_134973364.1 transporter substrate-binding domain-containing protein -
  CSB63_RS07185 (CSB63_1509) - 1355196..1355660 (-) 465 WP_134973366.1 8-oxo-dGTP diphosphatase -
  CSB63_RS07190 (CSB63_1510) uvrB 1355705..1357696 (-) 1992 WP_002953358.1 excinuclease ABC subunit UvrB -
  CSB63_RS07195 - 1357843..1358088 (-) 246 WP_255135202.1 hypothetical protein -
  CSB63_RS09955 - 1358384..1359321 (-) 938 Protein_1364 CPBP family intramembrane glutamic endopeptidase -
  CSB63_RS07210 (CSB63_1514) - 1359520..1361730 (+) 2211 WP_134973368.1 ABC transporter substrate-binding protein/permease -
  CSB63_RS07215 (CSB63_1515) - 1361730..1362470 (+) 741 WP_002945131.1 amino acid ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 655 a.a.        Molecular weight: 71828.84 Da        Isoelectric Point: 4.9881

>NTDB_id=634880 CSB63_RS06975 WP_134973353.1 1312742..1314709(-) (amiA) [Streptococcus thermophilus strain CH8]
MKLKKILGITGVALLSVGVLAACSSKSNTSGTTYRNVYSSDPETLDYITSIMGGTKAVLTNGVDGLMEADKYGNLVPSVA
EDWSVSKDGLTYTYKIRKGIKWYTSEGEEYANVTAKDFVTGLKHAADAKSGALYLVQDSIAGLSDYLSGANKDFSNVGVK
AIDDYTLQYTLKKQEPYWNSKTTYGVLFPVNEDFLKNKGKDFGKSTDPTSILYNGPFLLKSLTAKSSIELTKNENYWDKK
NVHFDSIKFSYSDGSDLESIERSFSDGALSIARVFPTSSNYASVEKKYKDNIFYTEPGASTSVIGVNIDRQSYKFSAKKT
DAEKTSTKKALLNKDFRQSINFAIDRTAYQSQVNGKDGAALALRNLFVPYDFVSAGDKTFGDLVTEKMSSYGDEWSGVNF
ADGQDGLYNDEKAKTEFAKAKEALQGEGVQFPVHLDLPVDQSSKLSVAQAQSLKQTIEKSLGSENVVIDINQMSSDDMQN
ATSNAANAAAEDWDISNGVGWGPDYQDPSTYLDILKTTSSENTKAFNGYDDPNNAAAAQVGLKDYDALLDSEASETTDIN
ARYDRYAQAQAWLEDSSLIIPLTVSNGAAPVISRLTPFTGASIQVGDKGSSYVKYVKSQEKVVTKKEYEQSREKWLKEKK
ASNEKAQKDLEKHIK

Nucleotide


Download         Length: 1968 bp        

>NTDB_id=634880 CSB63_RS06975 WP_134973353.1 1312742..1314709(-) (amiA) [Streptococcus thermophilus strain CH8]
ATGAAACTTAAAAAAATTCTTGGAATTACGGGTGTAGCCCTTCTAAGTGTAGGTGTGTTAGCGGCTTGTTCATCTAAATC
AAACACTAGCGGAACTACTTATCGTAACGTTTACAGTTCCGATCCAGAAACCTTGGATTATATTACATCAATTATGGGAG
GGACAAAAGCTGTTTTGACTAATGGTGTAGATGGTCTTATGGAAGCGGACAAATATGGTAATCTTGTTCCATCAGTAGCT
GAAGACTGGTCTGTGTCTAAAGATGGTCTTACTTATACCTATAAAATTCGTAAAGGGATCAAATGGTATACTTCTGAAGG
TGAAGAGTACGCTAATGTTACTGCTAAGGACTTTGTAACGGGTCTTAAGCATGCCGCTGATGCCAAATCTGGAGCCCTTT
ATTTGGTACAAGATTCTATTGCTGGATTGTCTGACTACCTTTCTGGTGCCAATAAAGATTTCTCAAATGTTGGCGTTAAA
GCAATAGACGATTACACTCTGCAATACACACTAAAAAAACAGGAACCATACTGGAACTCTAAGACAACCTATGGTGTGCT
CTTCCCAGTTAACGAAGACTTTCTTAAGAACAAAGGGAAAGATTTTGGTAAGTCAACAGACCCAACCTCTATCCTATATA
ACGGTCCTTTCCTTCTTAAATCTTTGACAGCTAAATCATCTATCGAGTTGACTAAGAATGAGAATTATTGGGATAAGAAA
AACGTGCATTTTGACTCTATCAAATTCTCATATTCTGATGGATCGGATCTAGAATCAATAGAACGTAGTTTCTCTGACGG
CGCTCTCAGCATTGCACGTGTCTTCCCTACGAGTTCAAACTATGCATCGGTAGAAAAAAAATATAAAGATAATATTTTTT
ACACAGAACCAGGTGCATCTACATCGGTTATTGGTGTAAACATTGACCGCCAAAGCTATAAATTCTCAGCTAAGAAGACA
GATGCTGAAAAGACATCAACTAAGAAGGCTCTTCTAAACAAAGACTTTCGTCAATCAATCAACTTCGCTATTGATCGTAC
AGCCTATCAATCACAAGTAAACGGTAAAGATGGCGCAGCTCTTGCTCTACGTAACCTTTTTGTTCCTTATGATTTTGTCA
GTGCAGGCGATAAGACCTTTGGTGATCTTGTGACAGAAAAAATGAGCTCTTATGGTGATGAGTGGTCTGGTGTTAACTTT
GCAGATGGTCAAGACGGTCTCTACAATGATGAAAAAGCTAAGACTGAGTTTGCCAAAGCCAAAGAGGCTCTTCAAGGAGA
AGGGGTACAATTCCCAGTTCACTTAGACCTTCCAGTTGATCAAAGTTCTAAACTTAGTGTTGCTCAAGCTCAATCTTTGA
AACAAACCATTGAAAAATCATTGGGTAGCGAAAATGTTGTTATAGATATTAACCAAATGTCATCTGATGATATGCAAAAT
GCGACTAGTAATGCGGCAAATGCTGCAGCAGAAGACTGGGATATTTCTAACGGTGTGGGTTGGGGTCCAGACTATCAAGA
TCCATCAACTTATCTTGATATCTTAAAAACAACATCAAGTGAAAATACCAAGGCTTTCAATGGTTATGATGATCCAAACA
ATGCAGCAGCAGCCCAAGTAGGTCTTAAGGATTATGATGCCTTGCTTGATTCAGAAGCTTCAGAAACAACAGACATCAAC
GCTCGTTACGACCGTTATGCTCAAGCCCAAGCTTGGTTGGAAGATAGCTCTCTTATCATTCCATTGACTGTTAGTAACGG
TGCAGCACCTGTTATCTCACGTTTGACACCATTTACGGGTGCTAGCATACAGGTGGGTGATAAGGGCTCATCCTACGTCA
AATATGTGAAATCACAAGAAAAAGTAGTTACTAAGAAAGAATATGAACAAAGTCGTGAAAAATGGCTTAAAGAAAAGAAA
GCATCTAACGAAAAAGCTCAAAAAGATCTTGAGAAACACATTAAATAA

Domains

Predicted by InterproScan.

Bacterial extracellular solute-binding proteins, family 5 Middle (SBP_bac_5)

(75-520)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiA Streptococcus salivarius strain HSISS4

89.024

100

0.892
  amiA3 Streptococcus thermophilus LMD-9

87.215

100

0.875
  amiA3 Streptococcus thermophilus LMG 18311

86.91

100

0.872