NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	LSG23_RS12985	Genome accession	NZ_CP088973
Coordinates	2660460..2661074 (-)	Length	204 a.a.
NCBI ID	WP_013352942.1	Uniprot ID	A0A411A874
Organism	Bacillus velezensis strain CMML20-16
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2655460..2666074

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LSG23_RS12955 (LSG23_12935)	rpsT	2655760..2656026 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
LSG23_RS12960 (LSG23_12940)	holA	2656043..2657084 (-)	1042	Protein_2507	DNA polymerase III subunit delta	-
LSG23_RS12965 (LSG23_12945)	-	2657124..2657278 (-)	155	Protein_2508	hypothetical protein	-
LSG23_RS12970 (LSG23_12950)	-	2657319..2657453 (+)	135	WP_003152870.1	YqzM family protein	-
LSG23_RS12975 (LSG23_12955)	comEC	2657472..2659823 (-)	2352	WP_077722611.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LSG23_RS12980 (LSG23_12960)	-	2659824..2660393 (-)	570	WP_003152868.1	ComE operon protein 2	-
LSG23_RS12985 (LSG23_12965)	comEA	2660460..2661074 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
LSG23_RS12990 (LSG23_12970)	comER	2661133..2661954 (+)	822	WP_014305449.1	late competence protein ComER	-
LSG23_RS12995 (LSG23_12975)	-	2662023..2662760 (-)	738	WP_031378935.1	class I SAM-dependent methyltransferase	-
LSG23_RS13000 (LSG23_12980)	rsfS	2662757..2663113 (-)	357	WP_003152864.1	ribosome silencing factor	-
LSG23_RS13005 (LSG23_12985)	yqeK	2663131..2663691 (-)	561	WP_077722612.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LSG23_RS13010 (LSG23_12990)	-	2663681..2664250 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
LSG23_RS13015 (LSG23_12995)	yhbY	2664261..2664551 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
LSG23_RS13020 (LSG23_13000)	aroE	2664545..2665387 (-)	843	WP_015417858.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22190.27 Da Isoelectric Point: 5.4841

                                >NTDB_id=634315 LSG23_RS12985 WP_013352942.1 2660460..2661074(-) (comEA) [Bacillus velezensis strain CMML20-16]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=634315 LSG23_RS12985 WP_013352942.1 2660460..2661074(-) (comEA) [Bacillus velezensis strain CMML20-16]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0634315