NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	LQE85_RS15495	Genome accession	NZ_CP088000
Coordinates	3365839..3366702 (+)	Length	287 a.a.
NCBI ID	WP_068853817.1	Uniprot ID	A0AAP6GZC8
Organism	Stenotrophomonas rhizophila strain Bl2-2
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3360839..3371702

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LQE85_RS15475 (LQE85_15475)	pilB	3361493..3363226 (-)	1734	WP_068853813.1	type IV-A pilus assembly ATPase PilB	Machinery gene
LQE85_RS15480 (LQE85_15480)	-	3363283..3363693 (-)	411	WP_249833008.1	pilin	-
LQE85_RS15485 (LQE85_15485)	pilA/pilAI	3363812..3364219 (-)	408	WP_068853815.1	pilin	Machinery gene
LQE85_RS15490 (LQE85_15490)	pilC	3364576..3365832 (+)	1257	WP_068853816.1	type II secretion system F family protein	Machinery gene
LQE85_RS15495 (LQE85_15495)	pilD	3365839..3366702 (+)	864	WP_068853817.1	A24 family peptidase	Machinery gene
LQE85_RS15500 (LQE85_15500)	coaE	3366710..3367318 (+)	609	WP_249833009.1	dephospho-CoA kinase	-
LQE85_RS15505 (LQE85_15505)	-	3367405..3368769 (-)	1365	WP_095363631.1	HAMP domain-containing sensor histidine kinase	-
LQE85_RS15510 (LQE85_15510)	-	3368732..3369409 (-)	678	WP_017356179.1	response regulator transcription factor	-
LQE85_RS15515 (LQE85_15515)	-	3369417..3369887 (-)	471	WP_095363630.1	hypothetical protein	-
LQE85_RS15520 (LQE85_15520)	rimK	3370120..3371025 (-)	906	WP_249833010.1	30S ribosomal protein S6--L-glutamate ligase	-

Sequence

Protein

Download Length: 287 a.a. Molecular weight: 31733.82 Da Isoelectric Point: 8.4749

                                >NTDB_id=632242 LQE85_RS15495 WP_068853817.1 3365839..3366702(+) (pilD) [Stenotrophomonas rhizophila strain Bl2-2]

MAFLDQHPGLGYPAAAALGLLIGSFLNVVILRLPKRLEWQWKRDAREVLELPDIYEPPPPGIVVEPSHCPHCKHKLSWYE

NIPLFSWLVLGGKCRHCHAPISIQYPLVELLTGLLVVASVWQFGFGWQGFGAIVLSCFLVALSGIDLRTQLLPDQLTLPL

MWLGLIGSIDNLYMPAKPALLGAIAGYLLLWSVWWLFKQITGKEGIGHGDFKLLAALGAWCGLKGILPIILLSSVLGAII

GSIWLYSRGRDRATPIPFGPYLALAGWIVFMWGEPLINAYLRVSGLR

Nucleotide

Download Length: 864 bp

                                >NTDB_id=632242 LQE85_RS15495 WP_068853817.1 3365839..3366702(+) (pilD) [Stenotrophomonas rhizophila strain Bl2-2]

ATGGCATTTCTCGACCAGCATCCCGGCCTCGGCTATCCGGCCGCGGCCGCACTGGGGTTGCTGATTGGCAGCTTCCTCAA

TGTGGTGATCCTGCGCCTGCCCAAGCGGCTGGAGTGGCAGTGGAAGCGCGACGCCCGCGAGGTGCTCGAGCTTCCGGACA

TCTACGAACCACCCCCGCCCGGGATCGTGGTCGAGCCGTCGCACTGCCCGCACTGCAAGCACAAGCTGTCCTGGTACGAG

AACATCCCGCTGTTCAGCTGGCTGGTCCTAGGCGGCAAGTGCCGCCACTGCCATGCGCCGATCTCCATCCAGTACCCGCT

GGTCGAGCTGTTGACCGGCTTGCTGGTGGTAGCCAGCGTCTGGCAGTTCGGCTTCGGCTGGCAGGGCTTCGGCGCCATCG

TGCTGAGCTGCTTCCTGGTGGCCTTGTCCGGTATCGACCTGCGCACCCAGTTGTTGCCTGACCAGCTCACCCTGCCGCTG

ATGTGGCTGGGCCTGATAGGCAGCATCGACAACCTGTACATGCCGGCCAAGCCTGCCCTGCTGGGCGCGATAGCCGGCTA

CCTGCTGCTGTGGTCGGTATGGTGGCTGTTCAAGCAGATCACCGGCAAGGAAGGCATTGGTCACGGCGATTTCAAGCTGC

TGGCCGCGCTGGGCGCCTGGTGCGGGTTGAAGGGCATCCTGCCGATCATCCTGCTCTCGTCGGTGCTTGGGGCGATCATC

GGTTCGATCTGGCTTTACTCGCGCGGCCGCGACCGTGCCACGCCGATTCCGTTCGGGCCCTACCTGGCCCTGGCGGGCTG

GATCGTGTTCATGTGGGGCGAGCCGTTGATCAACGCGTATCTGCGGGTGTCCGGCCTGCGCTGA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(133-241)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-123)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Acinetobacter baumannii D1279779	48.264	100	0.484
pilD	Acinetobacter nosocomialis M2	48.41	98.606	0.477
pilD	Vibrio campbellii strain DS40M4	46.479	98.955	0.46
pilD	Vibrio cholerae strain A1552	47.292	96.516	0.456
pilD	Neisseria gonorrhoeae MS11	44.043	96.516	0.425

Detailed information NTG0632242