Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   LOC77_RS13370 Genome accession   NZ_CP087957
Coordinates   2686283..2686897 (-) Length   204 a.a.
NCBI ID   WP_329957827.1    Uniprot ID   -
Organism   Bacillus velezensis strain B-001     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2681283..2691897
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOC77_RS13340 rpsT 2681583..2681849 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  LOC77_RS13345 holA 2681866..2682907 (-) 1042 Protein_2589 DNA polymerase III subunit delta -
  LOC77_RS13350 - 2682947..2683101 (-) 155 Protein_2590 hypothetical protein -
  LOC77_RS13355 - 2683142..2683276 (+) 135 WP_003152870.1 YqzM family protein -
  LOC77_RS13360 comEC 2683295..2685646 (-) 2352 WP_230639580.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LOC77_RS13365 - 2685647..2686216 (-) 570 WP_003152868.1 ComE operon protein 2 -
  LOC77_RS13370 comEA 2686283..2686897 (-) 615 WP_329957827.1 helix-hairpin-helix domain-containing protein Machinery gene
  LOC77_RS13375 comER 2686956..2687777 (+) 822 WP_014305449.1 late competence protein ComER -
  LOC77_RS13380 - 2687846..2688583 (-) 738 WP_014305450.1 class I SAM-dependent DNA methyltransferase -
  LOC77_RS13385 rsfS 2688580..2688936 (-) 357 WP_003152864.1 ribosome silencing factor -
  LOC77_RS13390 yqeK 2688933..2689514 (-) 582 WP_014305452.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  LOC77_RS13395 - 2689504..2690073 (-) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  LOC77_RS13400 yhbY 2690084..2690374 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  LOC77_RS13405 aroE 2690368..2691210 (-) 843 WP_003152856.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22256.35 Da        Isoelectric Point: 5.0355

>NTDB_id=632051 LOC77_RS13370 WP_329957827.1 2686283..2686897(-) (comEA) [Bacillus velezensis strain B-001]
MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=632051 LOC77_RS13370 WP_329957827.1 2686283..2686897(-) (comEA) [Bacillus velezensis strain B-001]
ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363