Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   N5856_RS18640 Genome accession   NZ_CP104613
Coordinates   3864422..3865081 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain AHM8C212RI     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3859422..3870081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5856_RS18625 (N5856_18560) ygiS 3861627..3863234 (-) 1608 WP_077039141.1 ABC transporter substrate-binding protein -
  N5856_RS18630 (N5856_18565) ygiV 3863343..3863825 (-) 483 WP_000183494.1 GyrI-like domain-containing protein -
  N5856_RS18635 (N5856_18570) ygiW 3863878..3864270 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  N5856_RS18640 (N5856_18575) ciaR 3864422..3865081 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  N5856_RS18645 (N5856_18580) qseC 3865078..3866427 (+) 1350 WP_000673355.1 quorum sensing histidine kinase QseC -
  N5856_RS18650 (N5856_18585) ygiZ 3866473..3866805 (-) 333 WP_094318002.1 DUF2645 family protein -
  N5856_RS18655 (N5856_18590) mdaB 3867163..3867744 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  N5856_RS18660 (N5856_18595) ygiN 3867775..3868089 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  N5856_RS18665 (N5856_18600) - 3868134..3869021 (-) 888 WP_094318001.1 MurR/RpiR family transcriptional regulator -
  N5856_RS18670 (N5856_18605) - 3869018..3869965 (-) 948 WP_001305988.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=631748 N5856_RS18640 WP_001221502.1 3864422..3865081(+) (ciaR) [Escherichia coli strain AHM8C212RI]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=631748 N5856_RS18640 WP_001221502.1 3864422..3865081(+) (ciaR) [Escherichia coli strain AHM8C212RI]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365