Detailed information NTG0631215

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LPS07_RS14555
• Genome accession: NZ_CP087716
• Coordinates: 3017252..3018370 (+)
• Length: 372 a.a.
• NCBI ID: WP_032063405.1
• Uniprot ID: -
• Organism: Acinetobacter pittii strain AP2044
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3012252..3023370

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LPS07_RS14545 (LPS07_14545)	yggS	3015376..3016068 (-)	693	WP_069119664.1	YggS family pyridoxal phosphate-dependent enzyme	-
LPS07_RS14550 (LPS07_14550)	pilT	3016195..3017232 (+)	1038	WP_002121749.1	type IV pilus twitching motility protein PilT	Machinery gene
LPS07_RS14555 (LPS07_14555)	pilU	3017252..3018370 (+)	1119	WP_032063405.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LPS07_RS14560 (LPS07_14560)	fur	3018440..3018877 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
LPS07_RS14565 (LPS07_14565)	omlA	3018989..3019387 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
LPS07_RS14570 (LPS07_14570)	-	3019452..3019766 (-)	315	WP_002121714.1	RnfH family protein	-
LPS07_RS14575 (LPS07_14575)	-	3019766..3020833 (-)	1068	WP_005075306.1	hypothetical protein	-
LPS07_RS14580 (LPS07_14580)	-	3020987..3021442 (-)	456	WP_002121698.1	bacteriohemerythrin	-
LPS07_RS14585 (LPS07_14585)	-	3021694..3022542 (-)	849	WP_014205980.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41965.17 Da
• Isoelectric Point: 6.6404

>NTDB_id=631215 LPS07_RS14555 WP_032063405.1 3017252..3018370(+) (pilU) [Acinetobacter pittii strain AP2044]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDFSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPEQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=631215 LPS07_RS14555 WP_032063405.1 3017252..3018370(+) (pilU) [Acinetobacter pittii strain AP2044]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAATAGTCTTTCAGGCGAAATTATTGGGCAACTCTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTATTAAGGCGAATTGAAACTAAAATTCCTTCAAT
TGATGACTTGCAATTACCGCCTGTGCTTAAAGATTTCTCAATGACCAAACGCGGTATCATTATTTTTGTAGGTGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCATATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACACGCTGGCTGCATCATTACCCAACGTGAAGTTGGGATCGATACAGACTCATTTGA
AATTGCTTTAAAGAATACGTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTTATGGACT
ACGCGATTGGCTTCGCCGAAACAGGTCACCTTGTATTAGCCACAATGCATGCGAACAATGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGCCACAGTCAGCTCTACATGGACTTATCTCTTAACTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCGCGCCGTGCCGCAATCGAGATTCTCATTAACTCACCATTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCGGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTGCG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGAGCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (32-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	53.514	99.462	0.532
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363