Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LP473_RS05275 Genome accession   NZ_CP087699
Coordinates   1052934..1054256 (-) Length   440 a.a.
NCBI ID   WP_003130658.1    Uniprot ID   A0A1V0NFR7
Organism   Lactococcus lactis subsp. lactis strain IBB417     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1047934..1059256
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LP473_RS05245 (LP473_05245) - 1049077..1050045 (+) 969 WP_003130649.1 PhoH family protein -
  LP473_RS05250 (LP473_05250) - 1050103..1050582 (+) 480 WP_003130650.1 NUDIX hydrolase -
  LP473_RS05255 (LP473_05255) ybeY 1050734..1051222 (+) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  LP473_RS05260 (LP473_05260) - 1051206..1051661 (+) 456 WP_003130653.1 diacylglycerol kinase -
  LP473_RS05265 (LP473_05265) - 1051675..1052253 (+) 579 WP_003130655.1 nucleotidyltransferase family protein -
  LP473_RS05270 (LP473_05270) comFC 1052287..1052691 (-) 405 WP_003130657.1 ComF family protein Machinery gene
  LP473_RS05275 (LP473_05275) comFA 1052934..1054256 (-) 1323 WP_003130658.1 DEAD/DEAH box helicase Machinery gene
  LP473_RS05280 (LP473_05280) - 1054313..1054942 (+) 630 WP_003130660.1 YigZ family protein -
  LP473_RS05285 (LP473_05285) - 1055076..1055573 (+) 498 WP_003130662.1 VanZ family protein -
  LP473_RS05290 (LP473_05290) - 1055621..1056640 (+) 1020 WP_003130663.1 DUF475 domain-containing protein -
  LP473_RS05295 (LP473_05295) - 1056756..1057502 (+) 747 WP_003130666.1 bifunctional metallophosphatase/5'-nucleotidase -
  LP473_RS05300 (LP473_05300) glyQ 1057787..1058740 (+) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50148.67 Da        Isoelectric Point: 10.0025

>NTDB_id=631046 LP473_RS05275 WP_003130658.1 1052934..1054256(-) (comFA) [Lactococcus lactis subsp. lactis strain IBB417]
MSTNQEKLFGRLLLKNDILQLIKNTDKISVSKIFSNFLLETKVNPILGMTSISSNKIKCNCCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYV
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=631046 LP473_RS05275 WP_003130658.1 1052934..1054256(-) (comFA) [Lactococcus lactis subsp. lactis strain IBB417]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTGCAACTTATAAAAAATACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAACGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACTGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTTTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATGTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACATCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(114-233)

Helicase conserved C-terminal domain (Helicase_C)

(300-403)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V0NFR7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.25

90.909

0.675
  comFA/cflA Streptococcus mitis SK321

51.759

90.455

0.468
  comFA/cflA Streptococcus pneumoniae Rx1

51.759

90.455

0.468
  comFA/cflA Streptococcus pneumoniae D39

51.759

90.455

0.468
  comFA/cflA Streptococcus pneumoniae R6

51.759

90.455

0.468
  comFA/cflA Streptococcus pneumoniae TIGR4

51.759

90.455

0.468
  comFA/cflA Streptococcus mitis NCTC 12261

51.005

90.455

0.461
  comFA Bacillus subtilis subsp. subtilis str. 168

36.585

100

0.375