Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LO769_RS05355 Genome accession   NZ_CP087600
Coordinates   1044923..1046245 (-) Length   440 a.a.
NCBI ID   WP_003130658.1    Uniprot ID   A0A1V0NFR7
Organism   Lactococcus lactis subsp. lactis strain IBB109     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1039923..1051245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LO769_RS05325 (LO769_05320) - 1041081..1042034 (+) 954 WP_023189344.1 PhoH family protein -
  LO769_RS05330 (LO769_05325) - 1042092..1042571 (+) 480 WP_003130650.1 NUDIX hydrolase -
  LO769_RS05335 (LO769_05330) ybeY 1042723..1043211 (+) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  LO769_RS05340 (LO769_05335) - 1043195..1043650 (+) 456 WP_003130653.1 diacylglycerol kinase -
  LO769_RS05345 (LO769_05340) - 1043664..1044242 (+) 579 WP_003130655.1 nucleotidyltransferase family protein -
  LO769_RS05350 (LO769_05345) comFC 1044276..1044680 (-) 405 WP_003130657.1 ComF family protein Machinery gene
  LO769_RS05355 (LO769_05350) comFA 1044923..1046245 (-) 1323 WP_003130658.1 DEAD/DEAH box helicase Machinery gene
  LO769_RS05360 (LO769_05355) - 1046302..1046931 (+) 630 WP_003130660.1 YigZ family protein -
  LO769_RS05365 (LO769_05360) - 1047065..1047562 (+) 498 WP_003130662.1 VanZ family protein -
  LO769_RS05370 (LO769_05365) - 1047610..1048629 (+) 1020 WP_003130663.1 DUF475 domain-containing protein -
  LO769_RS05375 (LO769_05370) - 1048739..1049491 (+) 753 WP_252172562.1 bifunctional metallophosphatase/5'-nucleotidase -
  LO769_RS05380 (LO769_05375) glyQ 1049776..1050729 (+) 954 WP_252172563.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50148.67 Da        Isoelectric Point: 10.0025

>NTDB_id=630356 LO769_RS05355 WP_003130658.1 1044923..1046245(-) (comFA) [Lactococcus lactis subsp. lactis strain IBB109]
MSTNQEKLFGRLLLKNDILQLIKNTDKISVSKIFSNFLLETKVNPILGMTSISSNKIKCNCCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYV
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=630356 LO769_RS05355 WP_003130658.1 1044923..1046245(-) (comFA) [Lactococcus lactis subsp. lactis strain IBB109]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTGCAACTTATAAAAAATACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAACGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACTGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTTTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATGTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACATCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(114-233)

Helicase conserved C-terminal domain (Helicase_C)

(300-403)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1V0NFR7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74.25

90.909

0.675
  comFA/cflA Streptococcus mitis SK321

51.759

90.455

0.468
  comFA/cflA Streptococcus pneumoniae Rx1

51.759

90.455

0.468
  comFA/cflA Streptococcus pneumoniae D39

51.759

90.455

0.468
  comFA/cflA Streptococcus pneumoniae R6

51.759

90.455

0.468
  comFA/cflA Streptococcus pneumoniae TIGR4

51.759

90.455

0.468
  comFA/cflA Streptococcus mitis NCTC 12261

51.005

90.455

0.461
  comFA Bacillus subtilis subsp. subtilis str. 168

36.585

100

0.375