Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   N5O74_RS21275 Genome accession   NZ_CP104509
Coordinates   4198606..4199265 (+) Length   219 a.a.
NCBI ID   WP_001221487.1    Uniprot ID   -
Organism   Escherichia coli strain E2     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4193606..4204265
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5O74_RS21250 (N5O74_21250) mqsA 4195291..4195686 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  N5O74_RS21255 (N5O74_21255) mqsR 4195688..4195984 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  N5O74_RS21260 (N5O74_21260) - 4196189..4196614 (-) 426 WP_222890937.1 GyrI-like domain-containing protein -
  N5O74_RS21265 (N5O74_21265) - 4196672..4197880 (-) 1209 WP_001339197.1 IS4-like element ISVsa5 family transposase -
  N5O74_RS21270 (N5O74_21270) ygiW 4198062..4198454 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  N5O74_RS21275 (N5O74_21275) ciaR 4198606..4199265 (+) 660 WP_001221487.1 quorum sensing response regulator transcription factor QseB Regulator
  N5O74_RS21280 (N5O74_21280) qseC 4199262..4200611 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  N5O74_RS21285 (N5O74_21285) ygiZ 4200657..4200989 (-) 333 WP_000917684.1 DUF2645 family protein -
  N5O74_RS21290 (N5O74_21290) mdaB 4201308..4201889 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  N5O74_RS21295 (N5O74_21295) ygiN 4201920..4202234 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  N5O74_RS21300 (N5O74_21300) parE 4202282..4204174 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24703.67 Da        Isoelectric Point: 6.9850

>NTDB_id=630112 N5O74_RS21275 WP_001221487.1 4198606..4199265(+) (ciaR) [Escherichia coli strain E2]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLLGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=630112 N5O74_RS21275 WP_001221487.1 4198606..4199265(+) (ciaR) [Escherichia coli strain E2]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACTAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361