Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   N2O69_RS03515 Genome accession   NZ_CP104274
Coordinates   724820..725479 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain Ec15103     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 719820..730479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N2O69_RS03490 parE 719911..721803 (+) 1893 WP_001774047.1 DNA topoisomerase IV subunit B -
  N2O69_RS03495 ygiN 721851..722165 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  N2O69_RS03500 mdaB 722196..722777 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  N2O69_RS03505 ygiZ 723096..723428 (+) 333 WP_000917685.1 DUF2645 family protein -
  N2O69_RS03510 qseC 723474..724823 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  N2O69_RS03515 ciaR 724820..725479 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  N2O69_RS03520 ygiW 725631..726023 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  N2O69_RS03525 ygiV 726076..726558 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  N2O69_RS03530 mqsR 726763..727059 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  N2O69_RS03535 mqsA 727061..727456 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  N2O69_RS03540 ygiS 727589..729196 (+) 1608 WP_001295629.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=627768 N2O69_RS03515 WP_001221493.1 724820..725479(-) (ciaR) [Escherichia coli strain Ec15103]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=627768 N2O69_RS03515 WP_001221493.1 724820..725479(-) (ciaR) [Escherichia coli strain Ec15103]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365