Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LK336_RS07295 Genome accession   NZ_CP086572
Coordinates   1491148..1492512 (-) Length   454 a.a.
NCBI ID   WP_031787934.1    Uniprot ID   A0A7U7JST8
Organism   Staphylococcus argenteus strain RIVM_M036020     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1486148..1497512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK336_RS07280 (LK336_07275) cysE 1487042..1487689 (-) 648 WP_072291959.1 serine O-acetyltransferase -
  LK336_RS07285 (LK336_07280) gltX 1488106..1489560 (-) 1455 WP_031787935.1 glutamate--tRNA ligase -
  LK336_RS07290 (LK336_07285) - 1490050..1491123 (-) 1074 WP_001028364.1 PIN/TRAM domain-containing protein -
  LK336_RS07295 (LK336_07290) radA 1491148..1492512 (-) 1365 WP_031787934.1 DNA repair protein RadA Machinery gene
  LK336_RS07300 (LK336_07295) - 1492989..1495445 (-) 2457 WP_000897144.1 ATP-dependent Clp protease ATP-binding subunit -
  LK336_RS07305 (LK336_07300) - 1495460..1496467 (-) 1008 WP_031787933.1 protein arginine kinase -
  LK336_RS07310 (LK336_07305) - 1496457..1497023 (-) 567 WP_000882064.1 UvrB/UvrC motif-containing protein -
  LK336_RS07315 (LK336_07310) - 1497042..1497503 (-) 462 WP_000551760.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49820.30 Da        Isoelectric Point: 7.0049

>NTDB_id=625548 LK336_RS07295 WP_031787934.1 1491148..1492512(-) (radA) [Staphylococcus argenteus strain RIVM_M036020]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPKHGVKTKEIAGKVQKLNSIKHETTPRVLTDSAEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKKKVLYITGEESLNQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
RVSRIEQRVQEAAKLGFKRAIIPKNNIGGWTYPEGIQVIGVTSVHEALSFALHS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=625548 LK336_RS07295 WP_031787934.1 1491148..1492512(-) (radA) [Staphylococcus argenteus strain RIVM_M036020]
TTGGCCAAGAAAAAAGTGATTTTTGAATGTATGGCTTGTGGTTATCAATCTCCTAAATGGATGGGGAAATGTCCCAATTG
TGGTGCTTGGAATCAAATGGAGGAAATTGTTGAAAAAGCTGCCAATCCAAAGCATGGAGTAAAAACAAAAGAAATAGCTG
GTAAAGTACAAAAATTAAATAGTATTAAACATGAAACGACACCTAGAGTATTAACGGATTCTGCAGAATTCAACCGCGTA
TTAGGTGGCGGTATTGTTAGCGGATCACTGGTCCTGATAGGTGGAGATCCAGGTATTGGTAAGTCAACGTTACTTTTACA
AATTTGTGCTTCTTTATCACAAAAGAAAAAAGTGTTATATATTACTGGGGAAGAATCCCTTAATCAAACGAAACTTCGAG
CAGAGCGTTTAGATGAAGACTCTAGTGAATTACAAGTATTAGCAGAAACAGATTTAGAAGTTATATATCAGACAGTTAAA
GAAGAACAACCAGACTTACTAGTAGTTGATTCTATTCAAACGATTTATCACCCTGAAATTAGTTCGGCACCGGGTTCTGT
TTCACAAGTTCGTGAAAGTACACAAAGTTTAATGAACATTGCTAAACAAATGAATATTGCAACTTTTATTGTTGGACATG
TAACGAAAGAAGGTCAAATTGCAGGTCCTAGATTATTGGAACATATGGTAGATACAGTGCTTTATTTTGAAGGTGATGAA
CATCATGCATATCGAATTTTACGTGCTGTTAAAAACCGTTTTGGCTCTACAAATGAAATGGGTATTTTTGAAATGAAACA
AAGCGGGTTAAAAGGTGTTAATAATCCGTCTGAAATGTTTTTAGAAGAACGTTCTACAAATGTTCCTGGGTCTACAATTG
TTGCAACAATGGAAGGTACAAGACCACTTTTAATAGAAGTACAAGCACTTGTGACACCGACAACATTTAATAATCCAAGA
AGAATGGCAACGGGAATTGATCATAATCGACTTAGTTTACTAATGGCTGTTTTAGAAAAGAAAGAAAATTATTTATTACA
GCAACAAGATGCTTATATCAAAGTTGCAGGTGGTGTGAAATTAACAGAGCCAGCTGTTGATTTAAGTGTAATTGTGGCTA
CCGCATCAAGCTTTAAAGATAAAGCAGTTGATGGTTTAGATTGTTACATTGGAGAAGTTGGTTTAACGGGTGAGGTACGT
CGCGTATCTCGGATAGAGCAGCGCGTACAAGAAGCTGCAAAGTTAGGCTTCAAACGTGCAATTATTCCTAAAAATAATAT
TGGCGGATGGACATATCCAGAAGGGATTCAAGTAATTGGCGTAACTTCTGTACATGAAGCACTATCATTCGCTCTACATT
CATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(77-218)

Subunit ChlI of Mg-chelatase (ChlI)

(353-426)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7JST8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.667

100

0.674
  radA Streptococcus pneumoniae Rx1

61.83

98.678

0.61
  radA Streptococcus pneumoniae D39

61.83

98.678

0.61
  radA Streptococcus pneumoniae R6

61.83

98.678

0.61
  radA Streptococcus pneumoniae TIGR4

61.83

98.678

0.61
  radA Streptococcus mitis NCTC 12261

61.83

98.678

0.61
  radA Streptococcus mitis SK321

61.83

98.678

0.61