NTDB

Overview

Name	ciaR	Type	Regulator
Locus tag	NYS59_RS22735	Genome accession	NZ_CP103789
Coordinates	4869020..4869691 (-)	Length	223 a.a.
NCBI ID	WP_003085524.1	Uniprot ID	A0A0H2Z6L5
Organism	Pseudomonas aeruginosa strain 2718
Function	repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 4864020..4874691

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NYS59_RS22710	-	4864793..4865245 (-)	453	WP_058149074.1	protein YgfX	-
NYS59_RS22715	-	4865229..4865483 (-)	255	WP_003085532.1	succinate dehydrogenase assembly factor 2	-
NYS59_RS22720	-	4865756..4866700 (+)	945	WP_003101947.1	YgfZ/GcvT domain-containing protein	-
NYS59_RS22725	-	4866801..4867637 (+)	837	WP_003085528.1	HDOD domain-containing protein	-
NYS59_RS22730	-	4867645..4869027 (-)	1383	WP_003085526.1	sensor histidine kinase	-
NYS59_RS22735	ciaR	4869020..4869691 (-)	672	WP_003085524.1	response regulator	Regulator
NYS59_RS22740	-	4869902..4871185 (+)	1284	WP_003134362.1	OprD family porin	-
NYS59_RS22745	-	4871215..4872198 (+)	984	WP_128652263.1	tripartite tricarboxylate transporter substrate binding protein	-
NYS59_RS22750	-	4872247..4872717 (+)	471	WP_023875774.1	tripartite tricarboxylate transporter TctB family protein	-
NYS59_RS22755	-	4872728..4874245 (+)	1518	WP_128652262.1	tripartite tricarboxylate transporter permease	-

Sequence

Protein

Download Length: 223 a.a. Molecular weight: 24431.24 Da Isoelectric Point: 5.1881

                                >NTDB_id=625447 NYS59_RS22735 WP_003085524.1 4869020..4869691(-) (ciaR) [Pseudomonas aeruginosa strain 2718]

MRILLVEDHPQLAESVVQALKGAGWTVDLLQDGVAADLALASEEYALAILDVGLPRMDGFEVLARLRGRGKTLPVLMLTA

RGEVKDRVHGLNLGADDYLAKPFELSELEARVKALLRRSVLGGEQLQRCGALVYDLGTRRFSLDEQPLTLTSREQAVLEA

MIARPGRVMSKEQLAAQVFGLDEEASADAIEIYVHRLRKKLEGGAVRIVTFRGLGYLLEAQGD

Nucleotide

Download Length: 672 bp

                                >NTDB_id=625447 NYS59_RS22735 WP_003085524.1 4869020..4869691(-) (ciaR) [Pseudomonas aeruginosa strain 2718]

ATGCGCATCCTTCTGGTGGAAGATCATCCGCAGCTCGCCGAAAGCGTGGTCCAGGCGCTCAAGGGCGCCGGCTGGACGGT

AGACCTGCTGCAGGACGGGGTGGCCGCCGACCTGGCCCTGGCCAGCGAGGAGTACGCCCTGGCGATTCTCGACGTCGGCC

TGCCGCGGATGGACGGCTTCGAGGTGCTGGCGCGCCTGCGCGGGCGCGGCAAGACCCTGCCGGTGCTGATGCTGACTGCG

CGCGGCGAGGTGAAGGACCGGGTGCACGGCCTCAATCTCGGCGCCGACGACTACCTGGCAAAGCCTTTCGAACTGTCCGA

GCTGGAGGCGCGGGTCAAGGCCCTGCTGCGCCGCAGCGTGCTCGGCGGCGAACAGTTGCAGCGCTGCGGCGCCCTGGTCT

ACGACCTCGGCACCCGGCGCTTCAGCCTCGACGAGCAGCCGCTGACCCTGACCTCGCGGGAACAGGCGGTGCTGGAAGCG

ATGATCGCCCGTCCGGGGCGGGTGATGAGCAAGGAGCAACTGGCCGCCCAGGTCTTTGGCCTGGACGAGGAGGCCAGCGC

CGACGCCATCGAGATCTACGTCCACCGCCTGCGCAAGAAGCTGGAAGGCGGCGCCGTGCGCATCGTCACCTTCCGCGGCC

TCGGCTACCTGCTGGAGGCGCAGGGTGACTGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(146-218)

Response regulator receiver domain (Response_reg)

(3-112)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0H2Z6L5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ciaR	Streptococcus pneumoniae Rx1	37.668	100	0.377
ciaR	Streptococcus pneumoniae D39	37.668	100	0.377
ciaR	Streptococcus pneumoniae R6	37.668	100	0.377
ciaR	Streptococcus pneumoniae TIGR4	37.668	100	0.377
covR	Lactococcus lactis subsp. lactis strain DGCC12653	37.668	100	0.377

Detailed information NTG0625447