Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   LMK00_RS04770 Genome accession   NZ_CP086395
Coordinates   975366..976622 (-) Length   418 a.a.
NCBI ID   WP_252175814.1    Uniprot ID   A0A9Q8Y3D5
Organism   Lactococcus formosensis strain I4/6O     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 970366..981622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMK00_RS04740 (LMK00_04740) - 971119..972225 (+) 1107 WP_252175812.1 alanine dehydrogenase -
  LMK00_RS04745 (LMK00_04745) - 972345..973304 (+) 960 WP_004257953.1 PhoH family protein -
  LMK00_RS04750 (LMK00_04750) - 973306..973779 (+) 474 WP_252175813.1 NUDIX hydrolase -
  LMK00_RS04755 (LMK00_04755) ybeY 973789..974277 (+) 489 WP_014024619.1 rRNA maturation RNase YbeY -
  LMK00_RS04760 (LMK00_04760) - 974261..974722 (+) 462 WP_096368379.1 diacylglycerol kinase family protein -
  LMK00_RS04765 (LMK00_04765) - 974725..975369 (-) 645 WP_096368378.1 ComF family protein -
  LMK00_RS04770 (LMK00_04770) comFA 975366..976622 (-) 1257 WP_252175814.1 DEAD/DEAH box helicase Machinery gene
  LMK00_RS04775 (LMK00_04775) - 976677..977300 (+) 624 WP_252175815.1 YigZ family protein -
  LMK00_RS04780 (LMK00_04780) - 977313..978335 (+) 1023 WP_019293557.1 DUF475 domain-containing protein -
  LMK00_RS04785 (LMK00_04785) - 978372..979394 (-) 1023 WP_252175816.1 alpha/beta hydrolase -
  LMK00_RS04790 (LMK00_04790) - 979712..980491 (+) 780 WP_096368375.1 formate/nitrite transporter family protein -
  LMK00_RS04795 (LMK00_04795) glyQ 980505..981473 (+) 969 WP_019292765.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 418 a.a.        Molecular weight: 47824.93 Da        Isoelectric Point: 10.1916

>NTDB_id=624748 LMK00_RS04770 WP_252175814.1 975366..976622(-) (comFA) [Lactococcus formosensis strain I4/6O]
MNELYGRLLLQKELTKIPDKAILFDGMKEVSKTIVMCNRCGKKSKKKEVYLPVGAYYCPHCIQMGHVRSDEKLYHLPQKN
FAAVSCLKWQGKLTGPQQYISDNLLNLHRQQKTVLVQAVTGAGKTEMIYQVIDATLKKGKAVGLTSPRIDVCLELYYRLQ
RDFSCPISLLHGKGEKYSRSPLVIATTHQLLRFRHAFDLLVLDEVDAFPFPDNEMLYFALAQARKPSSSLIYLTATTTNN
LEKQVKLGQIEKLQLPRRFHGFPLVLPQFFWQSKFYKMVKKQRKSGFPLLIFVPEIRLGEKLCQDLQTYFPHEEIAFVAS
TSLERLASVERFRQGKVSILVSTTILERGVTFPKVDVFVFQSHHHNFTSSSLIQIAGRVGRSTERPDGKVFFFHLGKTTA
MLEANKNIRNMNKAGEFL

Nucleotide


Download         Length: 1257 bp        

>NTDB_id=624748 LMK00_RS04770 WP_252175814.1 975366..976622(-) (comFA) [Lactococcus formosensis strain I4/6O]
ATGAATGAATTATACGGTAGACTTCTTCTGCAAAAAGAATTGACGAAGATACCAGACAAAGCCATACTTTTTGATGGTAT
GAAAGAAGTTTCAAAAACGATTGTCATGTGTAACCGCTGTGGTAAAAAGAGTAAGAAAAAAGAGGTTTATCTCCCCGTTG
GTGCTTATTATTGCCCCCATTGTATTCAGATGGGGCATGTTCGCTCTGATGAAAAACTTTATCACCTTCCACAGAAAAAT
TTTGCTGCAGTTTCTTGTCTAAAATGGCAGGGAAAGCTCACAGGTCCTCAGCAATATATCTCCGATAATTTACTTAATCT
GCATCGGCAGCAAAAAACAGTGCTGGTACAGGCTGTAACAGGGGCAGGAAAGACGGAAATGATTTATCAAGTCATTGATG
CCACCTTAAAGAAAGGAAAAGCTGTGGGTTTGACGAGTCCGCGTATTGATGTTTGTCTAGAACTTTACTATCGCTTACAG
AGGGATTTTTCCTGTCCCATCTCGCTTTTGCATGGAAAGGGTGAGAAATACAGCCGTTCCCCTCTTGTAATTGCAACCAC
ACATCAGCTTTTACGTTTTCGTCATGCCTTTGATTTACTGGTTCTCGACGAAGTTGACGCTTTTCCATTTCCAGATAATG
AAATGCTTTACTTTGCGCTGGCTCAAGCTCGAAAACCCTCTTCAAGCTTGATTTATCTTACTGCAACAACCACCAACAAT
TTGGAAAAACAAGTCAAGTTGGGACAAATCGAAAAGCTGCAACTTCCCCGACGCTTTCATGGTTTTCCACTAGTACTTCC
GCAATTTTTTTGGCAGAGCAAGTTTTACAAGATGGTCAAAAAACAACGAAAATCTGGTTTTCCATTACTTATCTTTGTCC
CTGAAATAAGACTAGGAGAAAAACTGTGCCAAGACTTACAAACCTATTTTCCTCATGAAGAAATTGCCTTTGTTGCATCA
ACGAGTCTTGAACGCTTAGCATCTGTGGAACGTTTTCGACAAGGAAAAGTTTCTATCCTTGTCTCAACGACTATTTTAGA
ACGTGGTGTAACCTTCCCTAAGGTTGACGTTTTTGTATTTCAAAGTCACCATCATAATTTCACAAGTTCAAGTCTCATTC
AAATTGCTGGACGAGTAGGCCGAAGTACTGAAAGACCTGATGGTAAGGTATTTTTCTTTCATTTAGGAAAAACAACAGCG
ATGTTGGAAGCCAATAAAAACATCAGAAATATGAATAAGGCTGGAGAATTCCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(93-204)

Helicase conserved C-terminal domain (Helicase_C)

(275-382)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

57.393

95.455

0.548
  comFA/cflA Streptococcus pneumoniae TIGR4

52.358

100

0.531
  comFA/cflA Streptococcus pneumoniae Rx1

52.358

100

0.531
  comFA/cflA Streptococcus pneumoniae D39

52.358

100

0.531
  comFA/cflA Streptococcus pneumoniae R6

52.358

100

0.531
  comFA/cflA Streptococcus mitis SK321

51.887

100

0.526
  comFA/cflA Streptococcus mitis NCTC 12261

50.943

100

0.517
  comFA Bacillus subtilis subsp. subtilis str. 168

37.215

100

0.39
  comFA Latilactobacillus sakei subsp. sakei 23K

38

95.694

0.364