Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   M5R22_RS03520 Genome accession   NZ_CP103742
Coordinates   716640..717299 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli O25b:H4-ST131 strain 2822     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 711640..722299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5R22_RS03490 (M5R22_03490) ygiN 712540..712854 (-) 315 WP_000633738.1 putative quinol monooxygenase -
  M5R22_RS03495 (M5R22_03495) mdaB 712885..713466 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  M5R22_RS03500 (M5R22_03500) - 713717..714196 (+) 480 WP_000069057.1 Hcp family type VI secretion system effector -
  M5R22_RS03505 (M5R22_03505) - 714199..714909 (+) 711 WP_000834026.1 hypothetical protein -
  M5R22_RS03510 (M5R22_03510) ygiZ 714916..715248 (+) 333 WP_000914696.1 DUF2645 family protein -
  M5R22_RS03515 (M5R22_03515) qseC 715294..716643 (-) 1350 WP_000673383.1 quorum sensing histidine kinase QseC -
  M5R22_RS03520 (M5R22_03520) ciaR 716640..717299 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  M5R22_RS03525 (M5R22_03525) ygiW 717451..717843 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  M5R22_RS03530 (M5R22_03530) ygiV 717896..718378 (+) 483 WP_000183485.1 GyrI-like domain-containing protein -
  M5R22_RS03535 (M5R22_03535) ygiS 718487..720094 (+) 1608 WP_001296417.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=624683 M5R22_RS03520 WP_001221493.1 716640..717299(-) (ciaR) [Escherichia coli O25b:H4-ST131 strain 2822]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=624683 M5R22_RS03520 WP_001221493.1 716640..717299(-) (ciaR) [Escherichia coli O25b:H4-ST131 strain 2822]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCTGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365