Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   M5T36_RS03435 Genome accession   NZ_CP103623
Coordinates   701377..702036 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 3985     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 696377..707036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T36_RS03410 (M5T36_03410) parE 696468..698360 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  M5T36_RS03415 (M5T36_03415) ygiN 698408..698722 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  M5T36_RS03420 (M5T36_03420) mdaB 698753..699334 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  M5T36_RS03425 (M5T36_03425) ygiZ 699653..699985 (+) 333 WP_000912120.1 DUF2645 family protein -
  M5T36_RS03430 (M5T36_03430) qseC 700031..701380 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  M5T36_RS03435 (M5T36_03435) ciaR 701377..702036 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  M5T36_RS03440 (M5T36_03440) ygiW 702188..702580 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  M5T36_RS03445 (M5T36_03445) ygiV 702633..703115 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  M5T36_RS03450 (M5T36_03450) parC 703661..705919 (+) 2259 WP_024249834.1 DNA topoisomerase IV subunit A -
  M5T36_RS03455 (M5T36_03455) plsC 706153..706890 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=623695 M5T36_RS03435 WP_001221502.1 701377..702036(-) (ciaR) [Escherichia coli strain 3985]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=623695 M5T36_RS03435 WP_001221502.1 701377..702036(-) (ciaR) [Escherichia coli strain 3985]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365