Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LJY09_RS00535 Genome accession   NZ_CP086208
Coordinates   99834..101210 (+) Length   458 a.a.
NCBI ID   WP_013054899.1    Uniprot ID   A0A8D4J4E8
Organism   Priestia sp. J2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 94834..106210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJY09_RS00515 - 95126..95587 (+) 462 WP_013054895.1 CtsR family transcriptional regulator -
  LJY09_RS00520 - 95629..96171 (+) 543 WP_028412656.1 UvrB/UvrC motif-containing protein -
  LJY09_RS00525 - 96175..97248 (+) 1074 WP_013054897.1 protein arginine kinase -
  LJY09_RS00530 clpC 97269..99710 (+) 2442 WP_014462041.1 ATP-dependent protease ATP-binding subunit ClpC -
  LJY09_RS00535 radA 99834..101210 (+) 1377 WP_013054899.1 DNA repair protein RadA Machinery gene
  LJY09_RS00540 disA 101214..102293 (+) 1080 WP_013054900.1 DNA integrity scanning diadenylate cyclase DisA -
  LJY09_RS00545 - 102441..103538 (+) 1098 WP_014462040.1 PIN/TRAM domain-containing protein -
  LJY09_RS00550 ispD 103564..104265 (+) 702 WP_028412657.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  LJY09_RS00555 ispF 104262..104741 (+) 480 WP_013054903.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49474.82 Da        Isoelectric Point: 6.9049

>NTDB_id=623695 LJY09_RS00535 WP_013054899.1 99834..101210(+) (radA) [Priestia sp. J2]
MAKRKTKFACQHCGYESAKWMGKCPGCGSWNSMVEEMEETKSSRRGAFSGATASKVQKPQSITAIESTTEPRIFTPSAEL
NRVLGGGIVRGSLVLIGGDPGIGKSTLLLQTSAQLAMKQNKVLYISGEESTKQTKLRADRLGVKAEELYVHAETNLELIL
EAISSMQPDFVVIDSIQTIYHADVTSAPGSVSQVRECTAELMRVAKTNGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEMGIFEMKESGLEEVLNPSEIFLEERSQGAAGSVVVASMEGTRPVLVELQALISPTSF
GNPRRMATGVDHNRVSLIMAVLEKRVGMLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDAASSATDVIVGEVGLT
GEVRRVSRIEQRVQEAVKLGFQRIIIPEKNLGGWKVPDGIDVIGVSTVAEALQYTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=623695 LJY09_RS00535 WP_013054899.1 99834..101210(+) (radA) [Priestia sp. J2]
ATGGCGAAACGAAAAACAAAATTTGCATGTCAGCATTGCGGATATGAATCCGCAAAATGGATGGGGAAATGTCCTGGATG
CGGAAGCTGGAATTCGATGGTGGAAGAAATGGAAGAAACGAAATCATCACGCCGAGGAGCTTTTTCCGGAGCTACTGCTT
CTAAAGTTCAAAAACCTCAGTCAATTACAGCGATTGAATCTACAACAGAACCACGGATTTTTACACCTTCTGCAGAGTTA
AACCGTGTATTAGGAGGCGGTATTGTCAGAGGGTCACTCGTTTTAATAGGGGGAGATCCGGGTATCGGAAAGTCTACTTT
ACTTTTGCAAACCTCCGCGCAGTTAGCAATGAAACAAAATAAAGTACTATATATTTCGGGAGAGGAATCAACCAAACAAA
CGAAGTTAAGAGCGGACAGGCTAGGTGTAAAAGCTGAAGAGCTTTATGTTCACGCAGAAACAAACTTAGAATTAATACTA
GAGGCCATCTCAAGCATGCAGCCTGATTTTGTGGTGATTGATTCAATTCAAACCATTTACCATGCTGATGTGACATCAGC
GCCAGGAAGCGTCTCACAGGTGCGAGAGTGTACGGCTGAACTAATGCGTGTTGCTAAAACGAATGGCATTGCCATCTTTA
TTGTGGGTCATGTAACGAAAGAAGGGGCCATTGCGGGTCCAAGACTTCTTGAGCATATGGTAGATACGGTTCTTTATTTT
GAAGGAGAGCGCCATCATACGTATCGTATATTAAGAGCGGTTAAGAACCGATTTGGATCTACAAATGAAATGGGCATTTT
TGAAATGAAAGAAAGTGGGCTAGAAGAAGTATTGAATCCATCTGAAATCTTTCTAGAAGAACGGTCTCAGGGAGCAGCAG
GTTCGGTTGTTGTCGCTTCAATGGAAGGGACAAGACCCGTATTAGTAGAGCTGCAGGCGCTAATCAGTCCAACGAGCTTT
GGAAACCCTAGAAGAATGGCGACAGGAGTAGATCATAATCGCGTTTCGCTTATCATGGCTGTCTTAGAAAAGCGTGTAGG
AATGCTGCTGCAGAATCAAGATGCTTATTTAAAAGTAGCAGGTGGAGTGAAATTAGATGAACCAGCAATCGATTTAGCTG
TAGCCGTTAGCATTGCTTCAAGCTTCAGAGATGCTGCTTCTAGCGCTACAGATGTTATTGTAGGAGAGGTAGGATTAACA
GGCGAAGTACGCCGAGTGTCTAGAATTGAACAGCGCGTTCAAGAAGCGGTAAAATTAGGGTTCCAACGAATTATTATTCC
GGAGAAAAATTTAGGAGGATGGAAAGTCCCAGATGGTATTGATGTGATTGGGGTATCAACAGTAGCGGAGGCTCTGCAAT
ATACATTAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

75.817

100

0.76
  radA Streptococcus pneumoniae Rx1

63.135

98.908

0.624
  radA Streptococcus pneumoniae D39

63.135

98.908

0.624
  radA Streptococcus pneumoniae R6

63.135

98.908

0.624
  radA Streptococcus pneumoniae TIGR4

63.135

98.908

0.624
  radA Streptococcus mitis NCTC 12261

63.135

98.908

0.624
  radA Streptococcus mitis SK321

63.135

98.908

0.624