Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   M5S83_RS20235 Genome accession   NZ_CP103609
Coordinates   4202430..4203089 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 4314     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4197430..4208089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5S83_RS20215 (M5S83_20215) plsC 4197576..4198313 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  M5S83_RS20220 (M5S83_20220) parC 4198547..4200805 (-) 2259 WP_001281841.1 DNA topoisomerase IV subunit A -
  M5S83_RS20225 (M5S83_20225) ygiV 4201351..4201833 (-) 483 WP_000183494.1 GyrI-like domain-containing protein -
  M5S83_RS20230 (M5S83_20230) ygiW 4201886..4202278 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  M5S83_RS20235 (M5S83_20235) ciaR 4202430..4203089 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  M5S83_RS20240 (M5S83_20240) qseC 4203086..4204435 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  M5S83_RS20245 (M5S83_20245) ygiZ 4204481..4204813 (-) 333 WP_000912120.1 DUF2645 family protein -
  M5S83_RS20250 (M5S83_20250) mdaB 4205132..4205713 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  M5S83_RS20255 (M5S83_20255) ygiN 4205744..4206058 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  M5S83_RS20260 (M5S83_20260) parE 4206106..4207998 (-) 1893 WP_000195292.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=623667 M5S83_RS20235 WP_001221502.1 4202430..4203089(+) (ciaR) [Escherichia coli strain 4314]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=623667 M5S83_RS20235 WP_001221502.1 4202430..4203089(+) (ciaR) [Escherichia coli strain 4314]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365