Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   M5T18_RS03550 Genome accession   NZ_CP103559
Coordinates   735368..736027 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain 3059     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 730368..741027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T18_RS03525 (M5T18_03525) - 731110..732057 (+) 948 WP_001305988.1 iron-siderophore ABC transporter substrate-binding protein -
  M5T18_RS03530 (M5T18_03530) - 732054..732941 (+) 888 WP_000614957.1 MurR/RpiR family transcriptional regulator -
  M5T18_RS03535 (M5T18_03535) ygiN 732986..733300 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  M5T18_RS03540 (M5T18_03540) mdaB 733331..733912 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  M5T18_RS03545 (M5T18_03545) qseC 734022..735371 (-) 1350 WP_000673373.1 quorum sensing histidine kinase QseC -
  M5T18_RS03550 (M5T18_03550) ciaR 735368..736027 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  M5T18_RS03555 (M5T18_03555) ygiW 736179..736571 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  M5T18_RS03560 (M5T18_03560) ygiV 736624..737106 (+) 483 WP_000183504.1 GyrI-like domain-containing protein -
  M5T18_RS03565 (M5T18_03565) parC 737652..739910 (+) 2259 WP_001586259.1 DNA topoisomerase IV subunit A -
  M5T18_RS03570 (M5T18_03570) plsC 740144..740881 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=623109 M5T18_RS03550 WP_001221493.1 735368..736027(-) (ciaR) [Escherichia coli strain 3059]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=623109 M5T18_RS03550 WP_001221493.1 735368..736027(-) (ciaR) [Escherichia coli strain 3059]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGTGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGTTAGCGGAACGTGTAGAGGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAACTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACTCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365